Antibiotic Susceptibility Profiles of Aeromonas hydrophila Isolates From Aquaculture Farms and Response to Potential Antibacterial Plant Extracts

Q3 Environmental Science

Asian Fisheries Science Pub Date : 2022-05-19 DOI:10.33997/j.afs.2022.35.2.004

L. T. Dang, Hanh Nguyen, Yen Pham, H. Truong

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引用次数: 0

Abstract

Aeromonas spp., which inhabits freshwater and marine water bodies, can be responsible for the diseases and mortalities of many different cultured fishes. In this study, the antibiotic susceptibility profiles of 20 Aeromonas hydrophila strains isolated from diseased freshwater fish cultured in the Red River Delta, Vietnam were categorised as non-wild-type (non-WT) strains or were resistant to at least one antibiotic. Also, in-vitro antibacterial activities of extracts from two local plants against several antibiotic-resistant A. hydrophila strains were done to screen for potential bio-antibiotic materials. The antibiotic susceptibility results showed that 25 % of bacterial strains were resistant to 3–9 antibiotics, 35 % to 2 antibiotics, and 10 % to one antibiotic. Both plants, rose myrtle seed, Rhodomyrtus tomentosa Hassk, 1842, extract and fermented garlic, Allium sativum Linnaeus, 1753, supernatant, showed inhibitory activities against antibiotic-resistant A. hydrophila strains. Furthermore, the R. tomentosa extract and the fermented A. sativum supernatant exhibited significant antibacterial effects to several A. hydrophila strains, namely A. hydrophila CEDMA17.021, CEDMA17.002, CEDMA17.008, and CEDMA17.009 resistant to two or more antibiotics. This study demonstrated multiple resistant profiles of A. hydrophila strains to different antibiotics and the inhibitory activities of R. tomentosa extract and the fermented A. sativum supernatant against antibiotic-resistant A. hydrophila strains. Hence, this study indicates the potential use of bio-antibiotics derived from plants to manage Aeromonas-related infections.

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水产养殖场嗜水气单胞菌分离株的药敏特征及对潜在抗菌植物提取物的反应

栖息在淡水和海洋水体中的气单胞菌可导致许多不同养殖鱼类的疾病和死亡。在这项研究中，从越南红河三角洲养殖的患病淡水鱼中分离的20株嗜水气单胞菌的抗生素敏感性谱被归类为非野生型(非wt)菌株或对至少一种抗生素耐药。同时，对两种当地植物提取物对几种耐药的嗜水单胞菌进行了体外抑菌活性研究，以筛选潜在的生物抗生素材料。药敏结果显示，25%的菌株对3 ~ 9种抗生素耐药，35%的菌株对2种抗生素耐药，10%的菌株对1种抗生素耐药。这两种植物，玫瑰桃金娘种子，Rhodomyrtus tomentosa Hassk, 1842，提取物和发酵大蒜，Allium sativum Linnaeus, 1753，上清，对耐药的嗜水芽孢杆菌菌株都有抑制活性。此外，绒毛毛霉提取物和发酵的sativum上清液对两种或两种以上抗生素耐药的嗜水单胞菌CEDMA17.021、CEDMA17.002、CEDMA17.008和CEDMA17.009均有显著的抑菌作用。本研究证实了嗜水单胞菌对不同抗生素的多重耐药谱，以及绒毛单胞菌提取物和发酵的sativum上清液对嗜水单胞菌耐药菌株的抑制活性。因此，这项研究表明，从植物中提取的生物抗生素可能用于控制气单胞菌相关感染。

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来源期刊

Asian Fisheries Science Agricultural and Biological Sciences-Food Science

CiteScore

2.20

自引率

0.00%

发文量

期刊介绍： The Asian Fisheries Science (AFS) was first published in 1987. It is an open access SCOPUS indexed publication of the Asian Fisheries Society. Four regular issues are published annually in March, June, September and December. In addition, special issues are published on specific topics. Full texts of the articles are available for free download and there is no publication fee. The journal promotes fisheries science which has an international appeal with special focus on Asian interests.