Antioxidative role of propolis on LPS induced renal damage

IF 1.2 Q3 MULTIDISCIPLINARY SCIENCES
Z. Doğanyiğit, B. Yakan, A. Okan, S. Silici
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引用次数: 2

Abstract

Abstract Sepsis is a systemic infectious disease that leads to shock, organ failure, and death and requires urgent treatment. Animal model studies of sepsis and endotoxemia have revealed that antioxidant compounds prevent the progression of multi-system organ failure and reduce death rate. In the present study aimed to establish the effect of propolis, which has been proven to have antibacterial, anti-inflammatory and antioxidant activities in recent years, on lipopolysaccharide (LPS)-induced renal damage. 40 Sprague dawley rats were randomly divided into five equal groups (n = 8): Control (0.9% NaCl), LPS (30 mg/kg), propolis (250 mg/kg), propolis + LPS, and LPS + propolis. After completion of the experimental protocol, Malondialdehyde (MDA) levels were measured using blood serum samples obtained from the rats. The kidney samples of the rats were examined histopathologically. As a result, it was determined that LPS increased MDA levels in the blood serum samples and it caused histological changes in the kidney tissue such as tubular damage, mild ischemic injury, ischemic damage in the form of vacuolization, tubular epithelial vacuolization, vascular congestion, and glomerular atrophy. Contrary to these results, MDA levels of serum decreased in the propolis + LPS, and LPS + propolis groups, and also histological findings improved. These results showed that protective effect of propolis against kidney damage caused by LPS.
蜂胶对LPS致肾损害的抗氧化作用
脓毒症是一种全身性感染性疾病,可导致休克、器官衰竭和死亡,需要紧急治疗。脓毒症和内毒素血症的动物模型研究表明,抗氧化剂化合物可以防止多系统器官衰竭的进展,降低死亡率。蜂胶近年来被证实具有抗菌、抗炎和抗氧化作用,本研究旨在探讨蜂胶对脂多糖(LPS)所致肾损伤的作用。选取40只大鼠,随机分为对照组(0.9% NaCl)、LPS (30 mg/kg)、蜂胶(250 mg/kg)、蜂胶+ LPS、LPS +蜂胶5组(n = 8)。实验方案完成后,使用从大鼠获得的血清样本测量丙二醛(MDA)水平。对大鼠肾脏标本进行组织病理学检查。结果确定LPS使血清样品中MDA水平升高,引起肾组织组织学改变,如肾小管损伤、轻度缺血性损伤、缺血性损伤表现为空泡化、肾小管上皮空泡化、血管充血、肾小球萎缩。与这些结果相反,蜂胶+ LPS组和LPS +蜂胶组血清MDA水平降低,组织学结果也有所改善。说明蜂胶对LPS致肾损害具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
The EuroBiotech Journal
The EuroBiotech Journal Agricultural and Biological Sciences-Food Science
CiteScore
3.60
自引率
0.00%
发文量
17
审稿时长
10 weeks
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