ASSESSMENT OF ANTICANCER ACTIVITY AND GENE EXPRESSION IN P53 GENE OF CISSUS QUADRANGULARIS L. STEM MEDIATED SILVER NANOPARTICLES

Abstract

Present study was conducted to evaluate the anticancer activity of Cissus quadrangularis stem mediated silver nanoparticles and its effect on the expression of p53 gene. In the current study the anticancer activity was assessed using the MTT assay on the MCF-7 breast cancer cell lines. In addition, the Quantitative Real Time PCR analysis was carried out to study the expression of p53 genes. The analysis revealed that, Cisplatin exhibits an IC50 value of 5.51 μg/ml when used as a positive control in MCF-7 cell line, whereas the silver nanoparticle extract at the same concentration exhibit an IC 50 value of 56.74 μg/ml. The MCF-7 breast cancer cells that were treated with the Cissus quadrangularis stem mediated silver nanoparticles showed down regulation of the p53 gene, going from 1.0 fold in control cells to 0.43 fold in the treated cells. The relative gene expression of the control is usually set to 1 in comparison to the test sample. In accordance with the MTT test results, the synthesized AgNPs have moderate anticancer efficacy. According to the findings of gene expression, MCF-7 cells treated with synthesized AgNPs from the stem of Cissus quadrangularis exhibit a 0.4564 fold decrease in the expression of p53 genes when compared to MCF-7 control cells.