Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence

IF 1.3 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
P. Ray, R. White
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引用次数: 13

Abstract

Cell-SELEX is performed to select for cell binding aptamers. We employed an additional selection pressure by using RNAse to remove surface-binding aptamers and select for cell-internalizing aptamers. A common RNA sequence was identified from independent cell-SELEX procedures against two different pancreatic cancer cell lines, indicating a strong selection pressure towards this sequence from the large pool of other available sequences present in the aptamer library. The aptamer is not specific for the pancreatic cancer cell lines, and a similar sequence motif is present in previously published internalizing aptamers. The identified sequence forms a structural motif that binds to a surface protein, which either is highly abundant or has strong affinity for the selected aptamer sequence. Deselecting (removing) this sequence during cell-SELEX may increase the probability of identifying aptamers against cell type-specific targets on the cell surface.
Cell SELEX鉴定“粘性”RNA适体序列
进行细胞SELEX以选择细胞结合适体。我们通过使用RNAse去除表面结合适体并选择细胞内化适体,采用了额外的选择压力。从针对两种不同的胰腺癌症细胞系的独立细胞SELEX程序中鉴定出一种常见的RNA序列,这表明从适体库中存在的大量其他可用序列中对该序列有强烈的选择压力。适体对胰腺癌症细胞系不是特异性的,并且类似的序列基序存在于先前发表的内化适体中。所鉴定的序列形成与表面蛋白结合的结构基序,表面蛋白对所选适体序列高度丰富或具有强亲和力。在细胞SELEX过程中去选择(去除)该序列可以增加识别针对细胞表面上的细胞类型特异性靶标的适体的概率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Nucleic Acids
Journal of Nucleic Acids BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.10
自引率
21.70%
发文量
5
审稿时长
12 weeks
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