Alpha-mangostin post-induction of lipopolysaccharides on interleukin-6 and bone sialoprotein expression in osteoblast cells: An experimental in vitro study

Abstract

Aim: Uncontrolled bone resorption after tooth extraction can cause a loss in alveolar bone dimension, which has an impact on alveolar bone preservation during dental implant treatment. Alpha-mangostin contained in mangosteen peel extract can reduce alveolar bone resorption while speeding up the bone formation process. This study determined whether the administration of α-mangostin decreases Interleukin-6 (IL-6) and increases Bone Sialoprotein (BSP) in the inflammatory process. Materials and Methods: The following four groups were subjected to an in-vitro assay to compare variations in the expression of inflammatory markers IL-6 and osteogenic markers BSP in LPS-induced cell line 7F2 cells before being administered α-mangostin. The bone sialoprotein (BSP), which is used as the osteogenesis marker of the effects of α-mangostin, is examined. Lipopolysaccharides (LPS) are employed in cell line 7F2 cultures of osteoblast cells to produce inflammatory effects. Real-time PCR was used to monitor the expression of genes related to inflammation and osteogenesis. Result: The Real-time PCR results showed that LPS can increase the expression of IL-6 by (15.12 ± 2.70), the administration of α-mangostin and LPS + α-mangostin can decrease the expression of IL-6 by (0.12 ± 0.04) and (0.25 ± 0.01). BSP expression increased in the provision of LPS and LPS + α-mangostin by (1.44 ± 0.33) and (3.13 ± 1.36), while in the administration of α-mangostin decreased by (0.96 ± 0.24). Conclusion: Alpha-mangostin has a potential effect to decrease the expression of the IL-6 gene and increase the expression of the BSP gene.