The Secondary Structural Models (16S rRNA) of Alkaline Amylase Producing Bacillus species strain SP-RM2: Phylogenetics Analysis

Abstract

Bacterial isolates especially the Bacillus strains have gained an edge over other organisms across the globe. Enzymes derived from alkaliphilic Bacillus make up about half of the total industrial enzyme market. Detergents, textiles, starch, baking and animal feed are the major industries which utmost produce and use enzymes. Alkaline α amylases are among the most important enzymes and are of great significance for biotechnology. In the present investigation, one alkalitolerant bacterial isolate coded RM2 was characterized by partial 16S rRNA gene sequencing due to its ability to produce alkaline amylase at pH 8.0. The amplified consensus 16S rRNA gene sequence (RM2) was further searched against nucleotide database using Blast program to find out its potential homologs. Twelve closest bacterial spp. were identified and the phylogenetic analysis revealed the target bacteria (RM2) isolate belong to genus Bacillus. RM2 has shown relatedness to Bacillus flexus strain IFO15715 in sequence based divergence study whereas secondary structure of 16S r-RNA of Bacillus sp. strain SPRM2 did not show any relatedness to Bacillus flexus strain IFO15715 even though they shared sequence based similarity.