Characterization and expression analysis of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) in embryonic development and immune response to bacteria

IF 0.7 4区生物学 Q4 MARINE & FRESHWATER BIOLOGY

Crustaceana Pub Date : 2023-01-31 DOI:10.1163/15685403-bja10259

Yi Chen Huang, Wei Kang Xu, H. Zhu, J. H. Chen

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引用次数: 0

Abstract

β-catenin is an important signal transduction protein in the canonical Wnt/β-catenin pathway, which is widely involved in the development of animals. However, the role of β-catenin in crustaceans is still unclear. In this study, the cDNA of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) was identified and characterized. The open reading frame (ORF) is 2424 bp, which encodes 807 amino acids containing an N-terminal region of a GSK-β consensus phosphorylation site and a central region of 11 armadillo (ARM) repeats. The tissue distribution showed that β-catenin was highly expressed in ovary, testis, hepatopancreas, heart, and gills. During embryonic development, the transcript of β-catenin was relatively high in the zygote, and the thirty-two-cell stage, blastula stage, gastrula stage, and nauplius stage. The transcripts of β-catenin in haemocytes, gills, and hepatopancreas were significantly up-regulated after infection with Vibrio anguillarum Bergeman, 1909. These results indicate that β-catenin participates in the regulation of embryonic development and in the immune response in E. carinicauda.

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Exopalaemon carinicauda (Holthuis, 1950) β-catenin在胚胎发育和细菌免疫应答中的表达分析

β-catenin是典型Wnt/β-catenin通路中重要的信号转导蛋白，广泛参与动物的发育过程。然而，β-连环蛋白在甲壳类动物中的作用尚不清楚。本研究对Exopalaemon carinicauda (Holthuis, 1950)中β-catenin的cDNA进行了鉴定和鉴定。开放阅读框(ORF)全长2424bp，编码807个氨基酸，包含GSK-β一致磷酸化位点的n端区域和11个armadillo (ARM)重复序列的中心区域。组织分布表明，β-catenin在卵巢、睾丸、肝胰腺、心脏和鳃中均有高表达。在胚胎发育过程中，β-catenin的转录量在合子、32细胞期、囊胚期、原胚期和无体期较高。在感染了鳗弧菌(Vibrio anguillarum Bergeman, 1909)后，血细胞、鳃和肝胰腺中β-catenin的转录量显著上调。这些结果表明，β-连环蛋白参与了牛毛蟹胚胎发育和免疫应答的调控。

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来源期刊

Crustaceana 生物-海洋与淡水生物学

CiteScore

1.30

自引率

33.30%

发文量

审稿时长

3 months

期刊介绍： Crustaceana is a leading journal in the world on crustacean research, including the latest papers from all branches of zoology. It provides up-to-date information on aspects such as taxonomy, zoogeography, ecology, physiology, anatomy, genetics, palaeontology, and biometry, and covers all groups of Crustacea. Boasting a large international circulation, Crustaceana provides its readers with an abstract for each article.