Identification of Vitellogenin 1 Potentially Related to Reproduction in the Cephalopod, Sepiella japonica

IF 1.3 4区农林科学 Q3 FISHERIES

Journal of Shellfish Research Pub Date : 2022-10-24 DOI:10.2983/035.041.0209

Liqin Liu, Shijie Zhao, Yao Zhang, Maoting Wang, Yunjun Yan, Zhengming Lü, L. Gong, Bingjian Liu, Yinghui Dong, Zhijin Xu

{"title":"Identification of Vitellogenin 1 Potentially Related to Reproduction in the Cephalopod, Sepiella japonica","authors":"Liqin Liu, Shijie Zhao, Yao Zhang, Maoting Wang, Yunjun Yan, Zhengming Lü, L. Gong, Bingjian Liu, Yinghui Dong, Zhijin Xu","doi":"10.2983/035.041.0209","DOIUrl":null,"url":null,"abstract":"ABSTRACT Vitellogenin (Vg) is the precursor of yolk protein, which plays an important role in the reproduction and development of oviparous animals. In this study, vitellogenin1 (Vg1) from Sepiella japonica was cloned and characterized, and its potential function in ovarian development was investigated. The full-length cDNAs of Vg1 was 5853 base pair (bp) that encoded 748 amino acids. Sequence analysis revealed that the overall protein sequence of Vg1 shared 66% identity with that of Uroteuthis edulis. Analyses with quantitative reverse transcription polymerase chain reaction (RT-qPCR) showed that the highest expression of Vg1 occurred in the ovary. As ovary developed, the expression of Vg1 increased in both ovary and liver from oogonia phase (I) to interstitial growth phase (III), and reached to the peak value at stage III. In addition, we examined the effects of Gonadotropin-releasing hormone (GnRH) on Vg1 expression in the ovary and liver were examined. The data showed that GnRH increased mRNA expression of Vg1, indicating that exogenous GnRH initially affected Vg1 expression and then influenced ovarian maturation. This study suggests that Vg1 might be involved in ovarian development in cephalopods.","PeriodicalId":50053,"journal":{"name":"Journal of Shellfish Research","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Shellfish Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2983/035.041.0209","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 1

Abstract

ABSTRACT Vitellogenin (Vg) is the precursor of yolk protein, which plays an important role in the reproduction and development of oviparous animals. In this study, vitellogenin1 (Vg1) from Sepiella japonica was cloned and characterized, and its potential function in ovarian development was investigated. The full-length cDNAs of Vg1 was 5853 base pair (bp) that encoded 748 amino acids. Sequence analysis revealed that the overall protein sequence of Vg1 shared 66% identity with that of Uroteuthis edulis. Analyses with quantitative reverse transcription polymerase chain reaction (RT-qPCR) showed that the highest expression of Vg1 occurred in the ovary. As ovary developed, the expression of Vg1 increased in both ovary and liver from oogonia phase (I) to interstitial growth phase (III), and reached to the peak value at stage III. In addition, we examined the effects of Gonadotropin-releasing hormone (GnRH) on Vg1 expression in the ovary and liver were examined. The data showed that GnRH increased mRNA expression of Vg1, indicating that exogenous GnRH initially affected Vg1 expression and then influenced ovarian maturation. This study suggests that Vg1 might be involved in ovarian development in cephalopods.

查看原文本刊更多论文

头足类动物日本海蛸生殖相关卵黄原蛋白1的鉴定

卵黄蛋白原（Vg）是卵黄蛋白的前体，在卵生动物的繁殖和发育中起着重要作用。本研究克隆并鉴定了日本Sepiella japonica卵黄蛋白1（Vg1），并对其在卵巢发育中的潜在作用进行了研究。Vg1的全长cDNA为5853个碱基对，编码748个氨基酸。序列分析表明，Vg1的蛋白质总序列与紫锥虫有66%的同源性。定量逆转录聚合酶链式反应（RT-qPCR）分析显示，Vg1在卵巢中的表达最高。随着卵巢的发育，从卵原细胞期（I）到间质生长期（III），Vg1在卵巢和肝脏中的表达均增加，并在III期达到峰值。此外，我们还检测了促性腺激素释放激素（GnRH）对Vg1在卵泡和肝脏中表达的影响。数据显示，GnRH增加了Vg1的mRNA表达，表明外源性GnRH最初影响Vg1的表达，然后影响卵巢成熟。本研究提示Vg1可能参与头足类动物的卵巢发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Shellfish Research 生物-海洋与淡水生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Original articles dealing with all aspects of shellfish research will be considered for publication. Manuscripts will be judged by the editors or other competent reviewers, or both, on the basis of originality, content, merit, clarity of presentation, and interpretations.