Visualized SERS Imaging of Single Molecule by Ag/Black Phosphorus Nanosheets

IF 31.6 1区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nano-Micro Letters Pub Date : 2022-03-15 DOI:10.1007/s40820-022-00803-x

Chenglong Lin, Shunshun Liang, Yusi Peng, Li Long, Yanyan Li, Zhengren Huang, Nguyen Viet Long, Xiaoying Luo, Jianjun Liu, Zhiyuan Li, Yong Yang

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引用次数: 29

Abstract

Highlights

Ag/BP-NS exhibit remarkable surface-enhanced Raman scattering performance with single-molecule detection ability. This remarkable enhancement can be attributed to the synergistic resonance enhancement of R6G molecular resonance, photo-induced charge transfer resonance and electromagnetic resonance.
A new polarization-mapping method was proposed, which can quickly screen out single-molecule signals and prove that the obtained spectra are emitted by single molecule.
The recognition of different tumor exosomes can be realized combining the method of machine learning.

AbstractSection Abstract

Single-molecule detection and imaging are of great value in chemical analysis, biomarker identification and other trace detection fields. However, the localization and visualization of single molecule are still quite a challenge. Here, we report a special-engineered nanostructure of Ag nanoparticles embedded in multi-layer black phosphorus nanosheets (Ag/BP-NS) synthesized by a unique photoreduction method as a surface-enhanced Raman scattering (SERS) sensor. Such a SERS substrate features the lowest detection limit of 10^–20 mol L⁻¹ for R6G, which is due to the three synergistic resonance enhancement of molecular resonance, photo-induced charge transfer resonance and electromagnetic resonance. We propose a polarization-mapping strategy to realize the detection and visualization of single molecule. In addition, combined with machine learning, Ag/BP-NS substrates are capable of recognition of different tumor exosomes, which is meaningful for monitoring and early warning of the cancer. This work provides a reliable strategy for the detection of single molecule and a potential candidate for the practical bio-application of SERS technology.

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银/黑磷纳米片单分子的可视化SERS成像

Ag/BP-NS具有优异的表面增强拉曼散射性能和单分子检测能力。这种显著的增强可归因于R6G分子共振、光诱导电荷转移共振和电磁共振的协同共振增强。提出了一种新的极化映射方法，该方法可以快速筛选出单分子信号，并证明得到的光谱是由单分子发射的。结合机器学习的方法可以实现对不同肿瘤外泌体的识别。摘要单分子检测与成像在化学分析、生物标志物鉴定等痕量检测领域具有重要的应用价值。然而，单分子的定位和可视化仍然是一个很大的挑战。在这里，我们报道了一种特殊工程的纳米结构，将银纳米颗粒嵌入多层黑磷纳米片(Ag/BP-NS)中，通过独特的光还原方法合成，作为表面增强拉曼散射(SERS)传感器。这种SERS底物对R6G的最低检出限为10-20 mol L−1，这是由于分子共振、光致电荷转移共振和电磁共振三种协同共振增强的结果。我们提出了一种偏振映射策略来实现单分子的检测和可视化。此外，结合机器学习，Ag/BP-NS底物能够识别不同的肿瘤外泌体，这对癌症的监测和早期预警具有重要意义。这项工作为单分子检测提供了可靠的策略，并为SERS技术的实际生物应用提供了潜在的候选方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano-Micro Letters NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

42.40

自引率

4.90%

发文量

715

审稿时长

13 weeks

期刊介绍： Nano-Micro Letters is a peer-reviewed, international, interdisciplinary and open-access journal that focus on science, experiments, engineering, technologies and applications of nano- or microscale structure and system in physics, chemistry, biology, material science, pharmacy and their expanding interfaces with at least one dimension ranging from a few sub-nanometers to a few hundreds of micrometers. Especially, emphasize the bottom-up approach in the length scale from nano to micro since the key for nanotechnology to reach industrial applications is to assemble, to modify, and to control nanostructure in micro scale. The aim is to provide a publishing platform crossing the boundaries, from nano to micro, and from science to technologies.