Pentachlorophenol Effect on Auxin Production by Pseudomonas fluorescens GU059580 and Its Application in Wastewater Bioremediation

Recent progress in materials Pub Date : 2022-11-18 DOI:10.21926/rpm.2204024

R. Werheni Ammeri, W. Hassen, I. Mehri, Nesrine Khelifi, A. Hassen

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Abstract

Bioaugmentation by Pseudomonas strains is widely used for the removal of pollutants in wastewater. In this study, we aimed to determine the removal of pentachlorophenol (PCP, 800 mg·L–1) in secondary wastewater by the bioaugmentation process. We determined the effects of using three surfactants, namely sodium dodecyl sulfate (SDS), cetyl-tri-methyl-ammonium bromide (CTAB), and Tween 80 for PCP removal. We determined the effect of the role of PCP surfactant for the biofilm and auxin production of the selected bacterial strain of P. fluorescens GU059580. High-performance liquid chromatography and spectroscopic analysis were used to determine PCP removal and bacterial growth, respectively. Biofilm production was determined using 96-well polystyrene plates, and auxin production was determined using spectrophotometric measurement at 535 nm. The results showed the removal of PCP from wastewater by P. fluorescens GU059580 is about 90.12%. The PCP removal from wastewater showed an improvement of about 96.5% after the addition of Tween 80, whereas significant biofilm formation was found in the mineral liquid medium supplemented with PCP and Tween 80, with a value of 3.78. The highest concentration of auxin was found in the presence of PCP without surfactants, showing a value of 1.7 mg·L–1. To conclude, P. fluorescens GU059580 can be used in bioreactors or some specific wastewater treatment processes for bioremediation.

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五氯酚对荧光假单胞菌GU059580产生长素的影响及其在废水生物修复中的应用

假单胞菌的生物强化作用广泛应用于废水中污染物的去除。在本研究中，我们旨在测定生物强化工艺对二次废水中五氯苯酚（PCP，800 mg·L–1）的去除率。我们测定了三种表面活性剂，即十二烷基硫酸钠（SDS）、十六烷基三甲基溴化铵（CTAB）和吐温80对PCP的去除效果。我们确定了PCP表面活性剂对所选荧光假单胞菌GU059580菌株的生物膜和生长素产生的作用。高效液相色谱法和光谱分析法分别用于测定PCP的去除和细菌生长。使用96孔聚苯乙烯板测定生物膜的产生，并且使用535nm的分光光度测量测定生长素的产生。结果表明，荧光假单胞菌GU059580对废水中PCP的去除率约为90.12%，添加吐温80后废水中PCP去除率提高约96.5%，而在添加PCP和吐温80的矿物液体培养基中，生物膜形成显著，生物膜值为3.78。在不含表面活性剂的PCP存在下，生长素浓度最高，显示值为1.7 mg·L–1。总之，荧光假单胞菌GU059580可用于生物反应器或一些特定的废水处理过程中进行生物修复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Recent progress in materials

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