Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes

Q4 Environmental Science

Colombia Forestal Pub Date : 2023-07-01 DOI:10.14483/2256201x.19696

Ana da Silva Lédo, Caroline de Araújo Machado, Annie Carolina Araújo de Oliveira, M. F. Arrigoni-Blank, Evaristo Mauro de Castro, A. V. Cruz da Silva

引用次数: 0

Abstract

Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4-Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA.

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汉鸟的愈伤组织发生和形态组织学特征

Hancornia speciosa Gomes属于夹竹桃科，分布在巴西的不同地区。本研究的目的是评价5份曼巴材料在不同体外培养条件下的愈伤组织诱导和组织分化。对来自曼加巴（巴西Embrapa）活性种质库的5个品种进行了评价。将体外发芽的植物幼苗用于切除不同的外植体（节间和节段，以及叶面部分）。将这些片段接种在含有不同浓度的6-苄基氨基嘌呤（BA）和2，4-二氯苯氧基乙酸（2，4-D）的培养基中。材料、处理和时间对愈伤组织质量（g）有显著影响。在缺乏调节剂的情况下没有愈伤组织的诱导。体外培养60天后，所有处理均表现出线性阳性细胞生长。在22.62µM 2,4-D和11.10µM BA的存在下，在BI登录中观察到最高的愈伤组织质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colombia Forestal Environmental Science-Nature and Landscape Conservation

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Colombia Forestal Journal publishes original manuscripts on themes of the forest field and various aspects of natural resources and the environment, which are discriminated in the categories of research article, review, reflection and technical notes of agreement with the stipulations of COLCIENCIAS for scientific publications. According to the classification of scientific areas of the Organization for Economic Cooperation and Development (OECD), Colombia Forestal Journal belongs to the large area of Agricultural Sciences (4), Agriculture, Forestry and Fisheries (4A) and to the discipline Forestry (4A02). Since its creation in 1978, the journal has been edited and published by the Francisco José de Caldas District University with headquarters in Bogotá. The quality of the content of the journal has been strengthened with the collaboration of national and international external evaluators in a double blind arbitration system and the dissemination has been extended by publishing manuscripts in a language different from Spanish and with inclusion in bibliographical databases such as Scopus. , DOAJ, Scielo, Redalyc, Ebsco, CAB Abstracts of CAB International, Forestry Abstracts, Ulrich''s, Latindex, Publindex. Likewise, it has academic profiles at Mendeley, Academia and Researchgate. The articles that are submitted to Colombia Forestal Journal must be original and unpublished and have not been postulated in other journals.