EDTA reduces cadmium toxicity in mustard (Brassica juncea L.) by enhancing metal chelation, antioxidant defense and glyoxalase systems

IF 2.1 Q2 PLANT SCIENCES

Acta Agrobotanica Pub Date : 2019-07-01 DOI:10.5586/AA.1772

J. Mahmud, M. Hasanuzzaman, K. Nahar, Anisur Rahman, M. Fujita

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引用次数: 20

Abstract

To investigate the possible role of EDTA in mitigating cadmium (Cd) toxicity, we treated mustard (Brassica juncea L.) seedlings with CdCl2 (0.5 mM and 1.0 mM, 3 days) alone and in combination with 0.5 mM EDTA in a semihydroponic medium. In the absence of EDTA, mustard seedlings accumulated Cd in their roots and shoots in a concentration dependent manner. Overaccumulation of Cd boosted generation of hydrogen peroxide (H2O2) and superoxide anions (O2•−), increased lipoxygenase (LOX) activity, lipid peroxidation, and cytotoxic methylglyoxal (MG) content. It also disturbed components of the antioxidant defense and glyoxalase systems. Furthermore, Cd stress decreased growth, leaf relative water content (RWC) and chlorophyll (chl) content but augmented the proline (Pro) content. On the other hand, EDTA supplemented Cd-stressed seedlings improved the constituents of the AsA-GSH cycle with the upregulated activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT). Moreover, addition of EDTA to the Cd-stressed seedlings notably enhanced Gly I activity in contrast to the stress treatment. Ethylenediaminetetraacetic acid decreased Cd accumulation in the both shoots and roots, as well as increased other nonprotein thiols (NPTs) in leaves, including the phytochelatin (PC) content. It also decreased H2O2 and O2•− generation, lipid peroxidation and MG content but enhanced RWC, chl and Pro contents in the leaves, which confirmed the improved growth of seedlings. The findings of the study suggest that exogenous application of EDTA to the Cd-treated seedlings reduces Cd-induced oxidative injuries by restricting Cd uptake, increasing NPTs concentration and upregulating most of the components of their antioxidant defense and glyoxalase systems.

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EDTA通过增强金属螯合、抗氧化防御和乙二醛酶系统降低芥菜中镉的毒性

为了研究EDTA在减轻镉（Cd）毒性中的可能作用，我们在半水培培养基中用CdCl2（0.5mM和1.0mM，3天）单独和与0.5mM EDTA联合处理芥菜（Brassica juncea L.）幼苗。在没有EDTA的情况下，芥菜幼苗的根和芽中镉的积累呈浓度依赖性。镉的过量积累促进了过氧化氢（H2O2）和超氧化物阴离子（O2•−）的产生，增加了脂氧合酶（LOX）活性、脂质过氧化和细胞毒性甲基乙二醛（MG）含量。它还扰乱了抗氧化防御和乙二醛酶系统的组成部分。此外，镉胁迫降低了生长、叶片相对含水量（RWC）和叶绿素（chl）含量，但增加了脯氨酸（Pro）含量。另一方面，补充EDTA的镉胁迫幼苗通过上调抗坏血酸过氧化物酶（APX）、单脱氢抗坏血酸还原酶（MDHAR）、脱氢抗坏血酸还原酶（DHAR）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性来改善AsA-GSH循环的组成。此外，与胁迫处理相比，向镉胁迫的幼苗中添加EDTA显著提高了Gly I活性。乙二胺四乙酸降低了Cd在地上部和根部的积累，并增加了叶片中的其他非蛋白硫醇（NPTs），包括植物螯合素（PC）含量。它还降低了叶片中H2O2和O2•−的生成、脂质过氧化和MG含量，但提高了叶片中RWC、chl和Pro的含量，这证实了幼苗生长的改善。研究结果表明，对镉处理的幼苗外源施用EDTA可以通过限制镉的吸收、增加NPTs的浓度以及上调其抗氧化防御和乙二醛酶系统的大部分成分来减少镉诱导的氧化损伤。

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来源期刊

Acta Agrobotanica Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.90

自引率

25.00%

发文量

审稿时长

16 weeks

期刊介绍： The Acta Agrobotanica publishes mainly significant, original research papers presenting the results new to the biology of cultivable or wild plants accompanying crops. The submissions dedicated particularly to flora and phytocenoses of anthropogenically transformed areas, bee pastures, nectariferous and polleniferous taxa, plant-pollinator relationships, urban and rural habitats for entomofauna, cultivated plants, weeds, aerobiology, plant pathogens and parasites are encouraged and accepted. Besides the original research papers, authors may submit short communications and reviews. The journal also publishes the invited papers in case of new developments in plant science. All submissions must be written in good English, which is solely a responsibility of the authors.