SELECTION OF AUTOCHThONOUS sACCHAROMYCES AND NON-sACCHAROMYCES YEASTS STRAINS ACCORDING TO THEIR EXTRACELLULAR ENZIMATIC ACTIVITY

E. Brȋnduşe, M. Ion, C. Nedelcu, Lidia Fȋciu, A. Pantazi
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Abstract

The aim of the present study was to investigate the production of extracellular enzymes in a number of twenty six autochtonous Saccharomyces and nonSaccharomyces strains selected in Dealu Mare region for wine production. The strains were screened for the production of extracellular b-glucosidase, esterase, pectinase and protease activity by inoculation the yeast strains onto selective media. All Saccharomyces tested strains showed at least two enzymatic activities while nonSaccharomyces strains showed activity at least for one enzyme. The weakest activity was recorded in case of β-glucosidase. Most of the tested strains exhibit more or less intense activity for polygalacturonase/pectinase and protease. This study put into evidence the potential of autochtonous and especially of non-Saccharomyces strains as source of production of secondary compounds which can play an important role in improving the quality of wines.
根据胞外酶活性筛选自身和非自身酵母菌株
本研究的目的是研究在迪卢马雷地区选择的26株自酿酒酵母和非酿酒酵母菌株中用于葡萄酒生产的胞外酶的生产。通过将酵母菌株接种到选择性培养基上,筛选出具有细胞外β-葡萄糖苷酶、酯酶、果胶酶和蛋白酶活性的菌株。所有测试的酿酒酵母菌株都显示出至少两种酶活性,而非酿酒酵母菌株显示出至少一种酶活性。β-葡萄糖苷酶的活性最弱。大多数测试菌株对多聚半乳糖醛酸酶/果胶酶和蛋白酶表现出或多或少的强烈活性。本研究证明了自酿酒酵母,尤其是非酿酒酵母菌株作为次生化合物生产来源的潜力,次生化合物在提高葡萄酒质量方面发挥着重要作用。
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