Growth inhibitory activity of Brassica oleracea var. Alboglabra on human gastric cancer cells

Abstract

Objectives: The aim of this study is to investigate anticancer activity of Brassica oleracea var. alboglabra (BOA) against the proliferation of BGC-823 human gastric cancer cells. Materials and Methods: B. oleracea var. alboglabra was extracted by ethanol 98% at a solid-to-liquid ratio of 1:8, (w/v) for 24h at room temperature. The cytotoxic effect of vegetables was examined by MTT assay. The migration of the cancer cells was conducted by wound healing assay and visualized under an inverted microscope. The mRNA expression level was quantified by real time PCR. Major Findings: It was found that ethanol extract of BOA exhibited the inhibitory activity against the proliferation of BGC-823 cells at IC50 value of 217.6 ± 2.8 µg/ml. Moreover, the treatment of BOA extract at concentration of 100 µg/ml for 24 h significantly suppressed the migration of gastric cancer cells into the gap as compared to the untreated cell group. Notably, the cytotoxic effect of BOA extract on human gastric cancer cells was found due to induction of apoptosis, mediating the up-regulation of caspase-8, -9, -3, and Bax in cancer cells. Conclusion: These results indicated that B. oleracea var. alboglabra have the potential inhibitory activity against the development of gastric cancer.