Growth inhibitory activity of Brassica oleracea var. Alboglabra on human gastric cancer cells

IF 0.7 Q4 PHARMACOLOGY & PHARMACY

Journal of Reports in Pharmaceutical Sciences Pub Date : 2022-07-01 DOI:10.4103/jrptps.jrptps_119_21

Dai-Hung Ngo, Hoang-Yen T Nguyen, Thi Nguyen, Dai-Nghiep Ngo, T. Vo

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引用次数: 0

Abstract

Objectives: The aim of this study is to investigate anticancer activity of Brassica oleracea var. alboglabra (BOA) against the proliferation of BGC-823 human gastric cancer cells. Materials and Methods: B. oleracea var. alboglabra was extracted by ethanol 98% at a solid-to-liquid ratio of 1:8, (w/v) for 24h at room temperature. The cytotoxic effect of vegetables was examined by MTT assay. The migration of the cancer cells was conducted by wound healing assay and visualized under an inverted microscope. The mRNA expression level was quantified by real time PCR. Major Findings: It was found that ethanol extract of BOA exhibited the inhibitory activity against the proliferation of BGC-823 cells at IC50 value of 217.6 ± 2.8 µg/ml. Moreover, the treatment of BOA extract at concentration of 100 µg/ml for 24 h significantly suppressed the migration of gastric cancer cells into the gap as compared to the untreated cell group. Notably, the cytotoxic effect of BOA extract on human gastric cancer cells was found due to induction of apoptosis, mediating the up-regulation of caspase-8, -9, -3, and Bax in cancer cells. Conclusion: These results indicated that B. oleracea var. alboglabra have the potential inhibitory activity against the development of gastric cancer.

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甘蓝对人胃癌细胞的生长抑制作用

目的：研究甘蓝型油菜（Brassica oleracea var.alboglabra，BOA）对BGC-823人癌症细胞增殖的抑制作用。材料与方法：以98%乙醇为提取液，固液比1:8，室温提取24小时。MTT法检测蔬菜的细胞毒作用。癌症细胞的迁移通过伤口愈合测定进行，并在倒置显微镜下观察。通过实时PCR对mRNA表达水平进行定量。主要发现：BOA乙醇提取物对BGC-823细胞增殖具有抑制作用，IC50值为217.6 ± 2.8µg/ml。此外，浓度为100µg/ml的BOA提取物处理24小时与未处理的细胞组相比，h显著抑制了癌症细胞迁移到间隙中。值得注意的是，发现BOA提取物对人癌症细胞的细胞毒性作用是由于诱导细胞凋亡，介导癌症细胞中胱天蛋白酶-8、-9、-3和Bax的上调。结论：马齿苋对癌症的发展具有潜在的抑制作用。

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来源期刊

Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.