Dynamic analysis of antibodies induced by leptospiral vaccines

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2019-11-30 DOI:10.3760/CMA.J.ISSN.0254-5101.2019.11.009

Ying Zhang, Yinghua Xu, Xiangyin Liu, Jinlong Zhang, Zhi-bin Chen, Guo-zhu Wang, Xiao-fang Xin, M. Zeng

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Abstract

Objective To investigate the dynamic changes of antibodies induced by leptospiral vaccines. Methods Antigens for antibody detection were screened out. ELISA was used to analyze antibody responses induced at different time points after immunizing guinea pigs with different batches of leptospiral vaccines from different manufacturers. To investigate the relationship between antibody responses induced by leptospiral vaccines and their protective effects in animal model, guinea pigs were challenged with Leptospira after immunization. Results There was no significant antigen-antibody reaction between the LigA protein or Patoc Ⅰ antigen and the serum samples of guinea pigs immunized with leptospiral vaccines. Notable IgG and IgM antibody reactions were observed in all vaccination groups when using bacterial proteins from seven Leptospira reference strains which were used for the preparation of leptospiral vaccines as envelope antigens. Antigen-specific IgG antibodies peaked at 35 d after the last immunization, and the highest peak of antigen-specific IgM antibodies was reached 11 d after the last immunization. Results of the challenge test showed that non-diluted leptospiral vaccines induced significant IgG and IgM antibody reactions in guinea pigs as compared with those diluted three or nine times, showing good protective effects. Conclusions Analysis of the dynamic changes of antibodies induced by leptospiral vaccines revealed that there was correlation between the induced serum antibody responses and the protective effects. This study provided reference for further study on alternative methods for evaluating leptospiral vaccine potency. Key words: Leptospira; Vaccine; Antibody; Protection

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钩端螺旋体疫苗诱导抗体的动态分析

目的探讨钩端螺旋体疫苗诱导抗体的动态变化。方法筛选用于抗体检测的抗原。ELISA用于分析不同制造商的不同批次钩端螺旋体疫苗免疫豚鼠后在不同时间点诱导的抗体反应。为了研究钩端螺旋体疫苗诱导的抗体反应与其在动物模型中的保护作用之间的关系，在免疫后用钩端螺旋病毒攻击豚鼠。结果LigA蛋白和PatocⅠ抗原与钩端螺旋体疫苗免疫豚鼠的血清样品无明显的抗原抗体反应。当使用用于制备钩端螺旋体疫苗的七个钩端螺旋参考菌株的细菌蛋白作为包膜抗原时，在所有疫苗接种组中观察到显著的IgG和IgM抗体反应。抗原特异性IgG抗体在最后一次免疫后35天达到峰值，抗原特异性IgM抗体在最后免疫后11天达到最高峰值。激发试验结果表明，与稀释三次或九次的疫苗相比，未稀释的钩端螺旋体疫苗在豚鼠中诱导了显著的IgG和IgM抗体反应，显示出良好的保护作用。结论钩端螺旋体疫苗诱导抗体的动态变化分析表明，诱导的血清抗体反应与保护作用之间存在相关性。本研究为进一步研究评估钩端螺旋体疫苗效力的替代方法提供了参考。关键词：钩端螺旋体；疫苗；抗体；保护

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.