Regulatory effect of mechanical vibration on fibroblast growth factor 23 antibody during fracture healing in ovariectomized rats

中华实验外科杂志 Pub Date : 2020-01-08 DOI:10.3760/CMA.J.ISSN.1001-9030.2020.01.034

Xue-hong Wang, Daming Sun, Dandan Zhou, Tingting Li, Yi-Ze Wang, Yongliang Hong, Kai Cheng

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Abstract

Objective To investigate the regulatory effect of mechanical vibration intervention on endocrine hormone fibroblast growth factor23 (FGF23) in ovariectomized fracture rats. Methods A model of postmenopausal osteoporosis was established in 45 female rats, and the animals were randomly divided into three groups: Sham operation group (Sham, n=15), ovariectomized fracture model group (OVX, n=15) and ovariectomized fracture vibration group (OVX-V, n=15). The rats in OVX-V group were subjected to whole-body vibration at a frequency of 35 Hz, a vibration amplitude of 2 mm, and an acceleration of 0.5 g, 20 min/d and 5 t/w, 5 days after fracture surgery. The rats in Sham group and OVX group were placed on the vibrating table for 20 min at 5th day after the operation, and no vibration was performed. Western blotting was used to detect the expression of FGF23 protein in the fracture end of rats. SPSS 19.0 statistical software was used to analyze the results. The results were expressed as mean±standard deviation (Mean±SD). Comparisons between groups were analyzed by one-way ANOVA. Results The protein expression of FGF23 in the fracture end of OVX-V group was significantly lower than that in OVX group at 2nd, 4th and 6th week [(0.846±0.012, 1.315±0.021, 1.315±0.021) vs. (0.703±0.009, 0.466±0.011, 0.380±0.005), P<0.01]. . The protein expression of FGF23 in the fracture end of OVX group was significantly higher than that in Sham group at 2nd, 4th and 6th week [(0.168±0.004, 0.321±0.003, 0.417±0.009) vs. (0.846±0.012, 1.315±0.021, 1.315±0.021), P<0.01), showing an increasing trend, suggesting that mechanical vibration can reduce the expression level of FGF23 in the bone tissue of oarotomy rats. Conclusion Mechanical vibration can reduce the expression of FGF23 in bone tissue, promote osteoblast differentiation, regulate bone mineralization and promote postmenopausal osteoporosis fracture healing. Key words: Mechanical vibration; Osteoporosis; Fracture; Endocrine hormones; Fibroblast growth factor23

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机械振动对去卵巢大鼠骨折愈合成纤维细胞生长因子23抗体的调节作用

目的探讨机械振动干预对去卵巢骨折大鼠内分泌激素成纤维细胞生长因子23 (FGF23)的调节作用。方法将45只雌性大鼠建立绝经后骨质疏松症模型，随机分为假手术组(Sham, n=15)、去卵巢骨折模型组(OVX, n=15)和去卵巢骨折振动组(OVX- v, n=15)。OVX-V组大鼠骨折术后5 d，全身振动频率35 Hz，振幅2 mm，加速度0.5 g, 20 min/d, 5 t/w。Sham组和OVX组于术后第5天置于振动台上20 min，不进行振动。Western blotting检测大鼠骨折端FGF23蛋白的表达。采用SPSS 19.0统计软件对结果进行分析。结果以均数±标准差(mean±SD)表示。组间比较采用单因素方差分析。结果OVX- v组骨折端FGF23蛋白表达在第2、4、6周明显低于OVX组[(0.846±0.012,1.315±0.021,1.315±0.021)比(0.703±0.009,0.466±0.011,0.380±0.005)，P<0.01]。OVX组骨折端FGF23蛋白表达在第2、4、6周显著高于Sham组[(0.168±0.004,0.321±0.003,0.417±0.009)比(0.846±0.012,1.315±0.021,1.315±0.021)，P<0.01]，呈升高趋势，提示机械振动可降低开腹大鼠骨组织中FGF23蛋白表达水平。结论机械振动可降低骨组织中FGF23的表达，促进成骨细胞分化，调节骨矿化，促进绝经后骨质疏松骨折愈合。关键词:机械振动;骨质疏松症;骨折;内分泌激素;成纤维细胞生长因子

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中华实验外科杂志

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