Revealing cryptic diversity and population divergence in subtropical aphids through DNA barcoding

IF 2.3 2区生物学 Q2 EVOLUTIONARY BIOLOGY

Zoologica Scripta Pub Date : 2023-09-01 DOI:10.1111/zsc.12613

Qiang Li, Qian-Xia Liu, Yuhua Yu, Xiaolan Lin, Xueyou He, Xiaolei Huang

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引用次数: 0

Abstract

Aphids are worldwide distributed pests, which feed on plant sap and cause serious losses to agricultural and forestry industries. Relatively few comprehensive studies have been conducted on aphid diversity in subtropics, although these regions may harbour hidden aphid diversity due to high plant diversity. In this study, we conducted DNA barcoding analysis for aphids in subtropical regions based on a comprehensive DNA barcode reference library including 5821 sequences and related ecological information (e.g. geography, host plant) of 379 morphospecies. In the reference library, 2140 sequences and related data of 151 morphospecies belonging to 77 genera and 11 subfamilies were newly produced by the current study. The average Kimura 2‐parameter distances within species, genera and subfamilies were 0.92%, 3.55% and 8.3%, respectively. The minimum interspecific distances were greater than the maximum intraspecific distances in 79.8% species, suggesting that barcode gaps existed in most aphid species. Automatic barcode gap discovery, Barcode Index Number, Bayesian Poisson tree processes and generalized mixed Yule‐coalescent revealed 419, 401, 462 and 455 single species‐representing clusters, respectively. A total of 32 morphospecies were assigned to more than one subclade respectively, suggesting geography or host plant‐mediated divergences and the existence of cryptic diversity. This study demonstrates that subtropical regions should have a higher species diversity of aphids, and cryptic species in certain morphospecies indicated in this study need to be investigated based on integrative taxonomic practices in the future. The DNA barcode reference library constructed herein provides a robust baseline data set to support future research in taxonomy, phylogenetics, ecology and evolution of aphids.

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用DNA条形码揭示亚热带蚜虫的隐性多样性和种群分化

蚜虫是分布在世界各地的害虫，以植物汁液为食，给农业和林业造成严重损失。尽管由于植物多样性高，亚热带地区可能隐藏着蚜虫的多样性，但对这些地区蚜虫多样性的综合研究相对较少。本研究基于379种形态种的5821条序列和相关生态信息(如地理、寄主植物等)，对亚热带蚜虫进行了DNA条形码分析。在参考文库中，本研究新增了11个亚科77属151种的2140个序列和相关数据。种、属和亚科间的木村2参数平均距离分别为0.92%、3.55%和8.3%。79.8%的蚜虫种间的最小种间距离大于种内的最大种间距离，表明大多数蚜虫种存在条形码间隙。自动条形码缺口发现、条形码索引、贝叶斯泊松树过程和广义混合Yule - coalescent分别发现了419个、401个、462个和455个单物种代表簇。共有32个形态种分别归属于一个以上的亚枝，表明地理或寄主植物介导的差异和隐多样性的存在。研究结果表明，亚热带地区蚜虫具有较高的物种多样性，未来需要在综合分类实践的基础上对某些形态种的隐种进行研究。本文构建的DNA条形码参考文库为今后蚜虫的分类、系统发育、生态学和进化等方面的研究提供了可靠的基础数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zoologica Scripta 生物-动物学

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Zoologica Scripta publishes papers in animal systematics and phylogeny, i.e. studies of evolutionary relationships among taxa, and the origin and evolution of biological diversity. Papers can also deal with ecological interactions and geographic distributions (phylogeography) if the results are placed in a wider phylogenetic/systematic/evolutionary context. Zoologica Scripta encourages papers on the development of methods for all aspects of phylogenetic inference and biological nomenclature/classification. Articles published in Zoologica Scripta must be original and present either theoretical or empirical studies of interest to a broad audience in systematics and phylogeny. Purely taxonomic papers, like species descriptions without being placed in a wider systematic/phylogenetic context, will not be considered.