Characterization of Cytokines and Proliferation Marker Ki-67 in Chronic Rhinosinusitis with Recurring Nasal Polyps

IF 1.8 Q3 RESPIRATORY SYSTEM

Advances in respiratory medicine Pub Date : 2022-10-01 DOI:10.3390/arm90050053

Rudolfs Janis Viksne, G. Sumeraga, M. Pilmane

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引用次数: 1

Abstract

Highlights What are the main findings? Cytokines are found in greater numbers in subepithelial connective tissue rather than epithelial cells in nasal polyps. IL-6 is an important factor in the pathogenesis of recurrent nasal polyps. What are the implications of the main finding? IL-6 was established as an important factor for further research in CRSwNP Possible future clinical implications, yet further research is necessary. Abstract Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammation of the mucosa of the nose and paranasal sinuses with the presence of polyps, affecting between 2.7% and 4.4% of the population. Cytokine analysis has become important in research on inflammatory mechanisms in CRSwNP. Therefore, our aim is to investigate the complex appearance, relative distribution, and interlinks of IL-1, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, and Ki-67 in CRSwNP. Methods: Samples of nasal polyps were obtained from 19 patients with previously diagnosed CRSwNP and the recurrence of polyps after previous surgeries. The control group consisted of samples from 17 otherwise healthy individuals with isolated nasal septum deviations. Tissues were stained for previously mentioned cytokines and Ki-67 immunohistochemically. Results: Polyp samples showed an increased presence of cytokines in subepithelial connective tissue and a decreased appearance in epithelium when compared to controls. There were several very strong, strong, and moderate correlations among factors. Conclusions: IL-6 strongly correlates with other cytokines as well as with the proliferation marker Ki-67, which suggests significant stimulation of this regulatory cytokine and its possible involvement in the pathogenesis of recurrent nasal polyps. IL-4, IL-7, IL-10, and IL-12 correlate with Ki-67, which suggests the possible involvement of these cytokines in tissue cell proliferation in the case of recurrent nasal polyps.

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慢性鼻窦炎伴复发性鼻息肉患者细胞因子及增殖标志物Ki-67的研究

亮点主要发现是什么？在鼻息肉中，上皮下结缔组织中的细胞因子数量比上皮细胞多。IL-6是复发性鼻息肉发病机制中的一个重要因素。主要发现的含义是什么？IL-6被确定为CRSwNP进一步研究的重要因素。可能的未来临床意义，但有必要进行进一步研究。摘要背景：慢性鼻窦炎伴鼻息肉（CRSwNP）是一种伴有息肉的鼻粘膜和鼻窦慢性炎症，影响2.7%至4.4%的人群。细胞因子分析在CRSwNP炎症机制的研究中具有重要意义。因此，我们的目的是研究CRSwNP中IL-1、IL-4、IL-6、IL-7、IL-8、IL-10、IL-12和Ki-67的复杂外观、相对分布和相互联系。方法：从19例既往诊断为CRSwNP和既往手术后息肉复发的患者中获取鼻息肉样本。对照组由17名患有孤立鼻中隔偏曲的健康个体的样本组成。对组织进行先前提到的细胞因子和Ki-67免疫组织化学染色。结果：与对照组相比，息肉样品显示上皮下结缔组织中细胞因子的存在增加，上皮中细胞因子出现减少。因素之间存在几个非常强、强和中等的相关性。结论：IL-6与其他细胞因子以及增殖标志物Ki-67密切相关，这表明这种调节性细胞因子的显著刺激及其可能参与复发性鼻息肉的发病机制。IL-4、IL-7、IL-10和IL-12与Ki-67相关，这表明这些细胞因子可能参与复发性鼻息肉的组织细胞增殖。

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来源期刊

Advances in respiratory medicine RESPIRATORY SYSTEM-

CiteScore

2.60

自引率

0.00%

发文量

期刊介绍： "Advances in Respiratory Medicine" is a new international title for "Pneumonologia i Alergologia Polska", edited bimonthly and addressed to respiratory professionals. The Journal contains peer-reviewed original research papers, short communications, case-reports, recommendations of the Polish Respiratory Society concerning the diagnosis and treatment of lung diseases, editorials, postgraduate education articles, letters and book reviews in the field of pneumonology, allergology, oncology, immunology and infectious diseases. "Advances in Respiratory Medicine" is an open access, official journal of Polish Society of Lung Diseases, Polish Society of Allergology and National Research Institute of Tuberculosis and Lung Diseases.