Antibiotic Resistance in Diarrheagenic Escherichia coli Isolated from Broiler Chickens in Pakistan

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2021-06-10 DOI:10.18502/jfqhc.8.2.6472

M. Amir, M. Riaz, Y-F Chang, Amir Ismail, A. Hameed, Muhammad Ahsin

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引用次数: 3

Abstract

Background: Diarrheagenic Escherichia coli (DEC) strains are predominant cause of gastrointestinal tract illnesses. The main objective of the study was to determine antibiotic resistance in various types of DEC isolated from chicken broilers farmed in Pakistan. Methods: A total of 200 feces and 200 meat samples from broiler chickens were collected from the slaughtering shops in Southern Punjab, Pakistan. The confirmed fecal (n=150) and meat (n=150) E. coli isolates were investigated against 16 antibiotics. Fourteen virulence genes specific for Enteropathogenic (EPEC), Shiga Toxin-producing (STEC), Enteroinvasive (EIEC), Enteroaggregative (EAEC), and Enterotoxigenic (ETEC) E. coli were identified using Polymerase Chain Reaction. Results: EPEC was the most detected pathotype in both feces (76%) and meat (90%) samples, followed by STEC, EIEC, and ETEC. The highest resistance (40-90%) was observed against penicillin, oxytetracycline, and nalidixic acid in fecal isolates. More than 50% EPEC and EAEC fecal isolates, and 60% EAEC meat isolates were simultaneously resistant to 6 or more antibiotics. Conclusion: Conclusively, the broiler meat sold in open markets of Pakistan was considerably contaminated with multi-drug resistant DEC. To mitigate the issue, the gov- ernment should regulate the use of antibiotics at poultry farms and monitor slaughtering practices in slaughterer houses.

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巴基斯坦肉鸡腹泻源性大肠杆菌的耐药性研究

背景：腹泻性大肠杆菌（DEC）菌株是引起胃肠道疾病的主要原因。本研究的主要目的是测定从巴基斯坦养殖的肉鸡中分离出的各种类型DEC的抗生素耐药性。方法：从巴基斯坦旁遮普邦南部屠宰场采集肉鸡粪便200份，肉制品200份。对已确认的粪便（n=150）和肉（n=50）大肠杆菌分离株进行了16种抗生素的研究。使用聚合酶链反应鉴定了14个对肠致病性（EPEC）、志贺毒素产生（STEC）、肠侵袭性（EIEC）、肠聚集性（EAEC）和产肠毒素（ETEC）大肠杆菌特异性的毒力基因。结果：EPEC在粪便（76%）和肉类（90%）中的检出率最高，其次是STEC、EIEC和ETEC。在粪便分离物中观察到对青霉素、土霉素和萘啶酸的最高耐药性（40-90%）。超过50%的EPEC和EAEC粪便分离株以及60%的EAEC肉分离株同时对6种或更多抗生素具有耐药性。结论：总之，巴基斯坦公开市场上出售的肉鸡肉受到了耐多药DEC的严重污染。为了缓解这一问题，政府应规范家禽养殖场抗生素的使用，并监督屠宰场的屠宰做法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.