The protective effect of co-administration of platelet-rich plasma (PRP) and pentoxifylline (PTX) on cyclophosphamide-induced premature ovarian failure in mature and immature rats

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2022-04-04 DOI:10.1080/15376516.2022.2057264

H. Aboutalebi, F. Alipour, A. Ebrahimzadeh-Bideskan

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引用次数: 2

Abstract

Abstract Cyclophosphamide (CP), as an antineoplastic agent, causes premature ovarian failure (POF) due to ovarian toxicity and subsequent infertility in women. Platelet-rich plasma (PRP) has accumulated significant attention in regenerative medicine. Pentoxifylline (PTX) as a methylxanthine derivative has been shown to have antioxidant and antiapoptotic properties. The aim of this study was to evaluate the protective effect of PRP and PTX on CP-induced POF. Fifty mature and immature female rats were assigned into five groups: control, CP (75 mg/kg, intraperitoneal [ip] on days 1 and 10 to induce POF), CP + PRP (200 μl, ip, half an hour after CP injection on day 1 and 10), CP + PTX (50 mg/kg, orally, half an hour after CP injection daily for 21 day), and CP + PRP + PTX. At the end of experiments on day 21, measurement of body weight, ovarian parameters (ovarian volume, follicular granulosa cell layers diameter, oocyte diameter, and the number of granulosa cells), measurement of ovarian hormone in sera for estradiol (E2), and anti-Mullerian hormone (AMH), as well as biochemical assessment were performed. The results showed that CP significantly reduced the ovarian parameters, E2, AMH, superoxide dismutase (SOD) activity and increased Malondialdehyde (MDA) levels compared to the control group (p < 0.001). Our results also indicated that all histomorphometric parameters and biochemical markers in CP-induced POF, were preserved close to normal by PRP and PTX treatments in both mature and immature rats (p < 0.001). Therefore, it is concluded that the co-administration of PRP and PTX can protect the ovary from CP-induced POF. Graphical Abstract

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富血小板血浆(PRP)和己酮茶碱(PTX)联合给药对环磷酰胺诱导的成熟和未成熟大鼠卵巢早衰的保护作用

摘要环磷酰胺(Cyclophosphamide, CP)是一种抗肿瘤药物，可导致卵巢毒性导致卵巢早衰(POF)，进而导致女性不孕。富血小板血浆(PRP)在再生医学中引起了广泛的关注。己酮可可碱(PTX)作为甲基黄嘌呤衍生物已被证明具有抗氧化和抗凋亡的特性。本研究的目的是评价PRP和PTX对cp诱导的POF的保护作用。将50只成熟和未成熟雌性大鼠分为5组:对照组、CP (75 mg/kg，腹腔注射[ip]，第1天和第10天诱导POF)、CP + PRP (200 μl, ip, CP注射后半小时，第1天和第10天)、CP + PTX (50 mg/kg，口服，CP注射后半小时，每天，连续21天)、CP + PRP + PTX。实验第21天结束时，测定大鼠体重、卵巢参数(卵巢体积、卵泡颗粒细胞层径、卵母细胞直径、颗粒细胞数)，测定血清中卵巢激素雌二醇(E2)、抗苗勒管激素(AMH)，并进行生化评价。结果表明，与对照组相比，CP显著降低卵巢参数、E2、AMH、超氧化物歧化酶(SOD)活性，升高丙二醛(MDA)水平(p < 0.001)。我们的研究结果还表明，PRP和PTX处理的成熟和未成熟大鼠POF的所有组织形态学参数和生化指标都保持接近正常(p < 0.001)。由此可见，PRP和PTX联合用药可保护卵巢免受cp诱导的POF。图形抽象

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.