Sirtuin1 and Sirtuin3 gene polymorphisms and acute myocardial infarction susceptibility

IF 0.8 Q4 GENETICS & HEREDITY
Mona Salah El-Din Habieb , Walaa Farid Abdel-Aziz , Abdel Hamid Abdo Ismail , Khadija Metwali Ahmed Sallam , Maathir Kamel El-Shafie
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引用次数: 2

Abstract

Objective

Acute myocardial infarction is one of the leading causes of death in the developed countries. This study aimed to study the association between sirtuin1 (SIRT1) gene polymorphism rs7069102 and promoter variant rs12293349 of sirtuin3 (SIRT3) gene and MI.

Patients and methods

150 subjects were enrolled: 100 myocardial infarction (MI) patients and 50 healthy individuals. All subjects were investigated for blood glucose, lipid profile, cardiac markers (creatine kinase (CK-MB) and troponin I (TnI) by ELISA and genotyping of SIRT1 rs7069102 and SIRT3 rs12293349 by real time PCR.

Results

MI cases had significantly higher prevalence of SIRT1 rs7069102 GG genotype and G allele when compared to controls. GG genotype and G allele elevated the risk of MI. The dominant (CG + GG versus CC) and recessive (GG versus CC + CG) models both showed a significant difference in the distribution of rs7069102 genotypes. Under the dominant model, total cholesterol, LDLc and CK-MB levels were significantly higher in CG + GG patients. While under the recessive model, GG patients showed significantly increased FBG, 2 h PPG, HbA1c, total cholesterol, and LDLc (p = 0.001). By univariate and multivariate logistic regression analysis, LDLc and total cholesterol were the only independent determining variables. Considering the SIRT3 rs12293349, there is no significant differences between patients and controls.

Conclusion

According to the findings, carrying the variant-type G allele of the SIRT1 rs7069102 was linked to an elevated risk of MI suggesting that this genetic variation could be a promising MI marker in an Egyptian population.

Sirtuin1和Sirtuin3基因多态性与急性心肌梗死易感性的关系
目的急性心肌梗死是发达国家的主要死亡原因之一。本研究旨在研究sirtuin1 (SIRT1)基因多态性rs7069102和sirtuin3 (SIRT3)基因启动子变异rs12293349与心肌梗死(MI)的关系。患者和方法共纳入150例受试者:100例心肌梗死(MI)患者和50例健康人群。采用ELISA检测所有受试者的血糖、血脂、心脏标志物(肌酸激酶(CK-MB)和肌钙蛋白I (TnI)),实时PCR检测SIRT1 rs7069102和SIRT3 rs12293349基因型。结果smi患者SIRT1 rs7069102 GG基因型和G等位基因的患病率明显高于对照组。GG基因型和G等位基因增加心肌梗死的风险,显性模型(CG + GG vs . CC)和隐性模型(GG vs . CC + CG) rs7069102基因型的分布均有显著差异。在优势模型下,CG + GG患者的总胆固醇、ldl和CK-MB水平明显升高。隐性模型下,GG患者FBG、2h PPG、HbA1c、总胆固醇、ldl显著升高(p = 0.001)。通过单因素和多因素logistic回归分析,ldl和总胆固醇是唯一的独立决定变量。考虑到SIRT3 rs12293349,患者与对照组之间无显著差异。根据研究结果,携带SIRT1 rs7069102变异型G等位基因与心肌梗死风险升高有关,这表明该遗传变异可能是埃及人群中有希望的心肌梗死标志物。
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来源期刊
Meta Gene
Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍: Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.
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