{"title":"Assessment of Oxytetracycline Residue in Table Eggs from Khartoum State’s Markets","authors":"Sara Am","doi":"10.34297/ajbsr.2021.14.001941","DOIUrl":null,"url":null,"abstract":"Background: Antibiotics residue in food from animal origin represents a public health issue concerns the authorities worldwide. Method: 100 egg samples were collected from Khartoum state’s markets to evaluate the current situation of oxy tetracycline residue. briefly, Samples were homogenized, weighed (2 g); and processed by adding citric acid (100 mg), 30% nitric acid (1 ml), methanol (4 ml) followed by adding 1 ml of deionized water; then centrifuged at 5300 for 10 mints followed by filtration throughout 0.22 µm syringe filter and 20 µl of the sample was injected into HPLC equipped with UV-VIS detector and ODS (C-18) column and read at 360 nm. Result: 49 % of total samples were positive. 48% of those positive samples (24 out of 49); were equal or exceeded the MRL of Oxytetracycline in egg (200 ppb). The mean values obtained from the three different state’s cities were not significantly different between different localities, never theless between each city and the MRL the variance was vastly significant.","PeriodicalId":93072,"journal":{"name":"American journal of biomedical science & research","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of biomedical science & research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34297/ajbsr.2021.14.001941","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Antibiotics residue in food from animal origin represents a public health issue concerns the authorities worldwide. Method: 100 egg samples were collected from Khartoum state’s markets to evaluate the current situation of oxy tetracycline residue. briefly, Samples were homogenized, weighed (2 g); and processed by adding citric acid (100 mg), 30% nitric acid (1 ml), methanol (4 ml) followed by adding 1 ml of deionized water; then centrifuged at 5300 for 10 mints followed by filtration throughout 0.22 µm syringe filter and 20 µl of the sample was injected into HPLC equipped with UV-VIS detector and ODS (C-18) column and read at 360 nm. Result: 49 % of total samples were positive. 48% of those positive samples (24 out of 49); were equal or exceeded the MRL of Oxytetracycline in egg (200 ppb). The mean values obtained from the three different state’s cities were not significantly different between different localities, never theless between each city and the MRL the variance was vastly significant.