Assessment of Oxytetracycline Residue in Table Eggs from Khartoum State’s Markets

Sara Am
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Abstract

Background: Antibiotics residue in food from animal origin represents a public health issue concerns the authorities worldwide. Method: 100 egg samples were collected from Khartoum state’s markets to evaluate the current situation of oxy tetracycline residue. briefly, Samples were homogenized, weighed (2 g); and processed by adding citric acid (100 mg), 30% nitric acid (1 ml), methanol (4 ml) followed by adding 1 ml of deionized water; then centrifuged at 5300 for 10 mints followed by filtration throughout 0.22 µm syringe filter and 20 µl of the sample was injected into HPLC equipped with UV-VIS detector and ODS (C-18) column and read at 360 nm. Result: 49 % of total samples were positive. 48% of those positive samples (24 out of 49); were equal or exceeded the MRL of Oxytetracycline in egg (200 ppb). The mean values obtained from the three different state’s cities were not significantly different between different localities, never theless between each city and the MRL the variance was vastly significant.
喀土穆州市场餐蛋中土霉素残留量的评估
背景:动物源性食品中抗生素残留是世界各地当局关注的公共卫生问题。方法:从喀土穆州市场采集100份鸡蛋样品,对土霉素残留现状进行评价。简单地说,将样品均化,称重(2g);并通过加入柠檬酸(100 mg)、30%硝酸(1 ml)、甲醇(4 ml),然后加入1 ml去离子水进行处理;然后在5300下离心10分钟,然后通过0.22µm注射器过滤器过滤,将20µl样品注入配备UV-VIS检测器和ODS(C-18)柱的HPLC中,并在360nm下读取。结果:49%的标本呈阳性。48%的阳性样本(49个样本中有24个);等于或超过土霉素在鸡蛋中的MRL(200ppb)。从三个不同州的城市获得的平均值在不同地区之间没有显著差异,即使每个城市与MRL之间的差异也非常显著。
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