2'-5'-Linked Triadenylates Act as Protein Kinase Activity Modulators

Abstract

2'-5'-linked triadenylates present the class of low molecular compounds, capable of regulating various cell functions. Their main function is the activation of ribonuclease L – the main enzyme of the innate immunity. Their dephosphorylation results in loss of this function, which, on the other hand grants them a handful of new, yet undiscovered abilities. Aim: Study the concentration-dependend influence of dephosphorylated 2'-5'-linked tradenylates on the protein kinases activity. Methods: Protein kinases were titrated with radioactively labeled ATP. After that the radioactivity value was determined with the use of scintillation counter. Results: We have found out that 2'-5'-linked are capable of changing the biological activity of various protein kinases by altering the amount of ATP they can potentially cleave. Conclusions: We suggest that this effect could occur to the specific changes in protein kinases structure.