Noggin proteins are multifunctional extracellular regulators of cell signaling

IF 3.3 3区 生物学
Genetics Pub Date : 2022-03-31 DOI:10.1093/genetics/iyac049
Prashath Karunaraj, O. Tidswell, E. Duncan, M. Lovegrove, Grace Jefferies, T. K. Johnson, C. Beck, P. Dearden
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引用次数: 0

Abstract

Abstract Noggin is an extracellular cysteine knot protein that plays a crucial role in vertebrate dorsoventral patterning. Noggin binds and inhibits the activity of bone morphogenetic proteins via a conserved N-terminal clip domain. Noncanonical orthologs of Noggin that lack a clip domain (“Noggin-like” proteins) are encoded in many arthropod genomes and are thought to have evolved into receptor tyrosine kinase ligands that promote Torso/receptor tyrosine kinase signaling rather than inhibiting bone morphogenic protein signaling. Here, we examined the molecular function of noggin/noggin-like genes (ApNL1 and ApNL2) from the arthropod pea aphid using the dorso-ventral patterning of Xenopus and the terminal patterning system of Drosophila to identify whether these proteins function as bone morphogenic protein or receptor tyrosine kinase signaling regulators. Our findings reveal that ApNL1 from the pea aphid can regulate both bone morphogenic protein and receptor tyrosine kinase signaling pathways, and unexpectedly, that the clip domain is not essential for its antagonism of bone morphogenic protein signaling. Our findings indicate that ancestral noggin/noggin-like genes were multifunctional regulators of signaling that have specialized to regulate multiple cell signaling pathways during the evolution of animals.
诺金蛋白是细胞信号传导的多功能细胞外调节因子
摘要Noggin是一种细胞外半胱氨酸结蛋白,在脊椎动物背腔模式中起着至关重要的作用。Noggin通过一个保守的N-末端片段结构域结合并抑制骨形态发生蛋白的活性。许多节肢动物基因组中编码了缺乏片段结构域的Noggin的非经典直向同源物(“Noggin-like”蛋白),并被认为已进化为受体酪氨酸激酶配体,促进Torso/受体酪氨酸激酶信号传导,而不是抑制骨形态发生蛋白信号传导。在这里,我们使用非洲爪蟾的背腹侧模式和果蝇的末端模式系统来检测节肢动物豌豆蚜的noggin/noggin样基因(ApNL1和ApNL2)的分子功能,以确定这些蛋白质是作为骨形态发生蛋白还是受体酪氨酸激酶信号调节因子。我们的研究结果表明,来自豌豆蚜的ApNL1可以调节骨形态发生蛋白和受体酪氨酸激酶信号通路,出乎意料的是,片段结构域对其拮抗骨形态发生蛋白质信号传导并不重要。我们的发现表明,祖先的noggin/noggin样基因是信号传导的多功能调节因子,在动物进化过程中专门调节多种细胞信号通路。
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来源期刊
Genetics
Genetics 生物-遗传学
CiteScore
6.20
自引率
6.10%
发文量
177
期刊介绍: GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.
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