Molecular evaluation of selected mango genotypes grown in Egypt using SCoT and ISSR markers

IF 1 Q3 HORTICULTURE

Horticultural Science Pub Date : 2022-12-22 DOI:10.17221/112/2021-hortsci

Ibrahim El-Shenawy Ghounim, Mohammed Raafat Mustafa, Ibrahim Hmmam

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引用次数: 0

Abstract

The mango (Mangifera indica L.) is a common tropical and subtropical fruit and the second main fruit crop in Egypt. In this work, start codon targeted (SCoT) and inter simple sequence repeat (ISSR) markers were employed to study the genetic relationship among twenty-three mango genotypes grown under the El-Giza district conditions. These genotypes consist of four newly introduced cultivars, six new progenies and thirteen closely related mango cultivars which were included as cultivar references for the best definition. Based on the overall SCoT and ISSR data, a total of 52 bands were generated (18 for SCoT and 34 for ISSR) with a total polymorphic percentage equal to 53.84% (44.44% for SCoT and 58.82% for ISSR). Based on the whole set of SCoT and ISSR marker data, the genetic similarity coefficients ranged from 80 to 97% with a mean equal to 88.5% and the ﬁrst two principal axes explained about 31% of the total genetic variation among the mango accessions. The SCoT and ISSR data revealed that the four newly introduced mango genotypes, namely ‘Aya’, ‘Kasturi’, ‘Maya’ and ‘Omer’, are novel genetic resources based on their genetic profiles, which can be used for future breeding programmes in Egypt.

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利用SCoT和ISSR标记对埃及芒果基因型的分子评价

芒果（Mangifera indica L.）是一种常见的热带和亚热带水果，是埃及的第二大水果作物。本工作采用起始密码子靶向（SCoT）和简单序列间重复（ISSR）标记研究了在El Giza地区条件下生长的23种芒果基因型之间的遗传关系。这些基因型由4个新引进的芒果品种、6个新后代和13个密切相关的芒果品种组成，这些品种被作为最佳定义的品种参考。根据SCoT和ISSR的总体数据，共产生52条带（SCoT 18条，ISSR 34条），总多态性百分比为53.84%（SCoT 44.44%，ISSR 58.82%）。基于整套SCoT和ISSR标记数据，遗传相似系数在80%至97%之间，平均值为88.5%，前两个主轴解释了芒果材料之间约31%的总遗传变异。SCoT和ISSR数据显示，四种新引入的芒果基因型，即“Aya”、“Kasturi”、“Maya”和“Omer”，是基于其遗传图谱的新遗传资源，可用于埃及未来的育种计划。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Horticultural Science Horticulture-园艺

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes results of basic and applied research from all areas of horticulture, fruit-growing, vegetable-growing, wine-making and viticulture, floriculture, ornamental gardening, garden and landscape architecture, concerning plants that are grown under the conditions of European temperate zone, or field plants that are considered as horticultural cultures. Original scientific papers, short communications and review articles are published in the journal. Papers are published in English (British spelling).