Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules

IF 2.6 3区生物学 Q2 PLANT SCIENCES

Journal of Plant Interactions Pub Date : 2023-09-04 DOI:10.1080/17429145.2023.2251511

A. Hidalgo-García, G. Tortosa, Pedro J. Pacheco, Andrew J. Gates, David J. Richardson, E. Bedmar, Lourdes Girard, M. J. Torres, María J. Delgado

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引用次数: 0

Abstract

Legumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N 2 O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity of Phaseolus vulgaris - Rhizobium etli symbiosis to emit N 2 O in response to nitrate (NO 3) has been demonstrated for the ﬁ rst time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and N 2 O formation in nodules. We also show that R. etli NarK is involved in NO 2-extrusion and links NO 3-reduction by NarB in the cytoplasm with NirK and cNor denitri ﬁ cation activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions

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普通根瘤菌通过同化硝酸盐还原与亚硝酸盐呼吸相结合，能够释放一氧化二氮

豆类可直接导致一些根瘤菌物种排放强效温室气体一氧化二氮（N2O），这些根瘤菌能够在自由生活条件下脱氮，并与植物共生。在这项研究中，首次证明了菜豆-根瘤菌共生体对硝酸盐（NO3）的反应释放N2 O的能力。我们发现，类杆菌同化硝酸还原酶（NarB）、亚硝酸盐还原酶（NirK）和一氧化氮还原酶（cNor）反硝化酶有助于根瘤中一氧化氮（NO）和N2O的形成。我们还表明，R.etli NarK参与了NO2的分泌，并将细胞质中NarB对NO3-的还原与周质中的NirK和cNor反硝化活性联系起来。这项工作中产生的知识将有助于探索农业土壤管理的战略和可持续做法，以提高豆类作物产量并减少温室气体排放

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来源期刊

Journal of Plant Interactions PLANT SCIENCES-

CiteScore

5.30

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Plant Interactions aims to represent a common platform for those scientists interested in publishing and reading research articles in the field of plant interactions and will cover most plant interactions with the surrounding environment.