Molecular prevalence of Porcine Circovirus 2 infection: foremost report in Southern states of India

IF 0.1 0 VETERINARY SCIENCES
P. S, R. A., A. K. Karuppannan, D. G, H. S., J. J, P. M, S. K, B. D, S. R., R. S., C. Ravishankar
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引用次数: 0

Abstract

: Porcine circovirus 2 (PCV2) is the emerging viral pathogen in the swine associated with multi-systemic clinical and subclinical outcomes. This study aimed to detect molecular and serological prevalence of PCV2 infection from southern states of India. A total of 434 random samples comprising of serum (n=273), pooled postmortem tissues (n=109) and rectal, vaginal and nasal swabs (n=52) and were collected from PCV2 suspected and healthy swine populations of Tamil Nadu, Kerala, Andhra Pradesh, Telangana and Puducherry states in India during 2019 to 2021 were screened for PCV2 by specific polymerase chain reaction (PCR) assay. Of 434 samples screened, 12.2% (n=53) showed positivity to PCV2 genome. Statistical analysis of molecular prevalence of PCV2 within breed, age, sex and vaccination status revealed no significant (p>0.05) difference but there was a significant (p<0.05) difference in the prevalence of PCV2 among healthy and suspected swine populations. Suspected pigs had significantly higher prevalence of PCV2 in comparison to healthy. ELISA based PCV2 antibody screening in 176 non-vaccinated serum samples revealed sero-positivity of 44.8% (n=79). The molecular and seroprevalence of PCV2 is alarming in southern states of India, which necessitates the need for genotypic characterization and phylogenetic analysis and development of candidate vaccine for implementation of suitable prevention and control measures.
猪圆环病毒2型感染的分子流行:印度南部各邦的首要报告
:猪圆环病毒2型(PCV2)是猪中新出现的与多系统临床和亚临床结果相关的病毒病原体。本研究旨在检测印度南部各州PCV2感染的分子和血清学流行率。2019年至2021年,共从印度泰米尔纳德邦、喀拉拉邦、安得拉邦、特伦甘纳邦和普杜切里邦的疑似和健康猪群中采集了434份随机样本,包括血清(n=273)、合并尸检组织(n=109)以及直肠、阴道和鼻拭子(n=52)。在筛选的434个样本中,12.2%(n=53)显示PCV2基因组阳性。对不同品种、年龄、性别和疫苗接种情况下PCV2分子流行率的统计分析显示,健康和疑似猪群中PCV2的流行率没有显著差异(p>0.05),但有显著差异(p<0.05)。与健康猪相比,疑似猪的PCV2患病率明显更高。在176份未接种疫苗的血清样本中,基于ELISA的PCV2抗体筛查显示血清阳性率为44.8%(n=79)。PCV2的分子和血清流行率在印度南部各州令人担忧,这就需要进行基因型表征和系统发育分析,并开发候选疫苗,以实施适当的预防和控制措施。
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
16
审稿时长
20 weeks
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