Infestation of Apis mellifera workers and larvae with Varroa destructor affects gut bacterial diversity

Q3 Agricultural and Biological Sciences

Journal of Entomological and Acarological Research Pub Date : 2022-08-01 DOI:10.4081/jear.2022.10316

F. Abuldahab, M. Abd-Elaziz, Y. El-Sayed, M. Abdallah, S. H. Mahmoud

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引用次数: 1

Abstract

The present work aims to investigate the midgut bacteria of honey bee (Apis mellifera lamarckii) workers and larvae infested with Varroa destructor. To achieve this task, uninfested and infested honey bee larvae and workers with the parasitic mites were obtained from natural bees apiaries in Qaliubiya Governorate, Egypt. Aerobic bacteria were isolated from the midgut of uninfested and infested A. mellifera on nutrient agar medium and identified using a culture-dependent isolation along with 16S rRNA sequences. The results revealed that both uninfested and V. destructor infested workers and larvae harbored gut bacteria belonging to Gamma-proteobacteria strains. The uninfested workers contained Enterobacter cloacae and Serratia nematodiphila, while V. destructor infested workers harbored Enterobacter species. Uninfested larvae contained E. hormaechei and Klebsiella variicola. V. destructor infested larvae harbored K. oxytoca as a result of infection. We concluded that the honeybee microbiome composition is likely to be impacted through isolates variation by stage and diversity disruption of isolates by V. destructor infection.

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华腐菌对蜜蜂工蜂和幼虫的感染影响肠道细菌多样性

本研究旨在研究蜜蜂工蜂和幼虫中肠细菌的分布情况。为了完成这项任务，在埃及Qaliubiya省的天然蜜蜂养蜂场采集了未感染和感染的蜜蜂幼虫和携带寄生螨的工蜂。在营养琼脂培养基上从未侵染和侵染的蜜蜂中肠中分离出需氧细菌，并利用培养依赖性分离和16S rRNA序列进行鉴定。结果表明，未侵染和被破坏弧菌侵染的工蜂和幼虫均含有γ -变形杆菌菌株。未感染的工蚁体内含有阴沟肠杆菌和嗜线虫沙雷菌，而被感染的工蚁体内含有肠杆菌。未受感染的幼虫含有甲伊氏杆菌和水痘克雷伯菌。由于受感染，被毁灭弧菌侵染的幼虫孳生了黑僵菌。我们认为，蜜蜂微生物组的组成可能是通过分离株的分期变化和V. destructor感染对分离株多样性的破坏而受到影响的。

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来源期刊

Journal of Entomological and Acarological Research Agricultural and Biological Sciences-Insect Science

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Entomological and Acarological Research (JEAR), formerly the Bollettino di Zoologia Agraria e di Bachicoltura of the Institute of Entomology of the Università degli Studi, Milano, was founded in 1928 by Remo Grandori. Thereafter, Minos Martelli and Luciano Süss hold the direction of the Journal until December 2011. In January 2012 the Editor decided for the new open-access on-line version of JEAR. The Journal publishes original research papers concerning Arthopods, but reviews, editorials, technical reports, brief notes, conference proceeding, letters to the Editor, book reviews are also welcome. JEAR has four main areas of interest: -Entomology (systematics; morphology; biology; biotechnology; agriculture, ornamental and forest entomology; applied entomology; integrated pest management; biological control; apiculture and apidology; medical, urban and veterinary entomology; etc.) -Stored product pests (biology; integrated pest management; etc.) -Insect Ecology (behaviour; biodiversity; taxonomy; plant insect interaction and ecosystems; biological control; alien species; etc.) -Acarology (systematics; morphology; biology; parasitology; control; etc.) The publication of manuscripts is subject to the approval of the Section Editor who has knowledge of the field discussed in the manuscript in accordance with the principles of Peer Review; referees will be selected from the Editorial Board or among qualified scientists of the international scientific community. Articles must be written in English and must adhere to the guidelines and details contained in the Instructions to Authors.