Design of functional vancomycin-embedded bio-derived extracellular matrix hydrogels for repairing infectious bone defects

IF 6.1 3区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nanotechnology Reviews Pub Date : 2023-01-01 DOI:10.1515/ntrev-2022-0524

Qingshuang Dong, Sunfang Chen, Jiuqin Zhou, Jingcheng Liu, Yubin Zou, Jia-Feng Lin, Jun Yao, D. Cai, Danhua Tao, Bing Wu, Bin Fang

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引用次数: 1

Abstract

Abstract The treatment of infectious bone defects has become a troublesome issue in orthopedics. The disease requires effective anti-infective and bone-reconstruction therapeutic functionalities. In this study, we prepared a novel antibacterial material (vancomycin-impregnated periosteal extracellular matrix [Van-PEM]) by embedding vancomycin in a periosteal extracellular matrix (PEM)-derived hydrogel via physical stirring for the treatment of infectious bone defects. The microstructure, porosity, degradation, and release properties of this antibacterial hydrogel were characterized. The in vitro hemolytic reaction, cytotoxicity, osteogenic ability, and antibacterial properties were also carefully studied. The results showed that the Van-PEM hydrogel possessed a fibrous network structure with high porosity. Moreover, the hydrogel demonstrated slow degradation in vitro and could release vancomycin for at least 1 week. The hydrogel showed no cytotoxicity and possessed good biocompatibility with blood cells. It also promoted osteogenesis and exerted a significant bactericidal effect. Subsequently, the anti-infection and bone-healing abilities of the antibacterial hydrogel were investigated in a rat model of infectious calvarial defects, and the infectious skull defect was successfully cured in vivo. Therefore, Van-PEM hydrogels may represent a promising therapeutic approach for treating infectious bone defects.

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功能性万古霉素包埋生物衍生细胞外基质水凝胶修复感染性骨缺损的设计

摘要感染性骨缺损的治疗已成为骨科的一个难题。这种疾病需要有效的抗感染和骨重建治疗功能。在本研究中，我们通过物理搅拌将万古霉素包埋在骨膜细胞外基质（PEM）衍生的水凝胶中，制备了一种新型抗菌材料（万古霉素浸渍的骨膜细胞外基体[Van PEM]），用于治疗感染性骨缺损。对该抗菌水凝胶的微观结构、孔隙率、降解和释放性能进行了表征。还仔细研究了体外溶血反应、细胞毒性、成骨能力和抗菌性能。结果表明，Van PEM水凝胶具有高孔隙率的纤维网络结构。此外，水凝胶在体外降解缓慢，可以释放万古霉素至少1周。该水凝胶无细胞毒性，与血细胞具有良好的生物相容性。它还能促进成骨，并发挥显著的杀菌作用。随后，在感染性颅骨缺损大鼠模型中研究了抗菌水凝胶的抗感染和骨愈合能力，并在体内成功治愈了感染性颅骨缺陷。因此，Van PEM水凝胶可能是治疗感染性骨缺损的一种很有前途的治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanotechnology Reviews CHEMISTRY, MULTIDISCIPLINARY-NANOSCIENCE & NANOTECHNOLOGY

CiteScore

11.40

自引率

13.50%

发文量

137

审稿时长

7 weeks

期刊介绍： The bimonthly journal Nanotechnology Reviews provides a platform for scientists and engineers of all involved disciplines to exchange important recent research on fundamental as well as applied aspects. While expert reviews provide a state of the art assessment on a specific topic, research highlight contributions present most recent and novel findings. In addition to technical contributions, Nanotechnology Reviews publishes articles on implications of nanotechnology for society, environment, education, intellectual property, industry, and politics.