Nano Magnetic Beads Combined with Polymerase Chain Reaction Detection of 2019-nCoV and Analysis of the Epidemiological and Clinical Characteristics of 2019-nCoV Patients

Abstract

The 2019 novel coronavirus (2019-nCoV) is extremely infectious and the human population globally is generally susceptible to it Its spread poses a serious threat to public health Analyzing the epidemiological and clinical characteristics of patients with coronavirus disease 2019 (COVID-19) can more fully demonstrate its characteristics and evolution, and provide effective scientific strategies for preventing and controlling the epidemic In this study, nucleic acid detection was performed on 228 suspected COVID-19 cases in Ningbo City from January 2020 to August 2020 The RNA of the virus was extracted by the nano magnetic bead method and was detected by real-time fluorescent quantitative PCR Patients whose test results were positive were considered as research subjects Clinical data of the patients were collected through a medical record system and the epidemiology and characteristics of COVID-19 were analyzed Among the 228 suspected cases, 108 patients had a positive nucleic acid test result The 108 confirmed patients were selected as research subjects, including 9 patients with mild symptoms, 85 patients with common symptoms, and 14 patients with severe symptoms The age range of the patients was 17-82 years Overall, 35 patients (32 40%) had complications The median incubation period for patients was 7 5 days Seventy patients (64 81%) had a contact history with a diseased individual In terms of the clinical symptoms of the patients, the following symptoms were identified: fever in 92, cough in 62, fatigue in 34, sputum expectoration in 32, dry cough in 22, sore throat in 19, diarrhea in 8, headache in 8, shortness of breath in 7, nasal congestion in 5, and muscle ache in 5 cases The results of chest CT imaging showed that 96 patients (88 89%) exhibited bilateral or unilateral pneumonia lesions of varying degrees, manifested as bilateral or unilateral lung patches, cloudy floccules, patches with an increased density shadow The CT images of 12 patients (11 11%) showed no obvious abnormalities, while 24 cases (22 22%) had absolute white blood cell count of 10x10(9)/L The absolute neutrophil count in 10 patients (9 26%) increased (>6 3x10(9/)L) The absolute lymphocyte count in 49 patients (45 37%) decreased (10 mg/L);14 (12 96%) had elevated D-dimer (>256 mu g/L);14 (12 96%) had high levels of alanine aminotransferase (>40 U/L);9 (8 33%) had increased aspartate aminotransferase (>40 U/L);while 32 (29 6%) had increased lactate dehydrogenase (>250 U/L) COVID-19 in Ningbo is mainly transmitted in clusters, and elderly patients are more likely to develop severe symptoms There is no significant difference in clinical characteristics between patients with severe conditions and those with mild and common ones