Biodecolorization of Remazol Brilliant Blue–R dye by Tropical White-Rot Fungi and Their Enzymes in The Presence of Guaiacol

S. Anita, F. Ningsih, D. Yanto
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Abstract

The ability of the tropical white-rot fungi and their enzyme to decolorize synthetic dyes was investigated. Production of lignin-modifying enzymes (LMEs) from the three new isolated fungi, namely Trametes hirsuta D7, Ceriporia sp. BIOM 3, and Cymatoderma dendriticum WM01 were observed for 9 days incubation under static condition. The results showed that the LMEs production enhanced in the present of guaiacol. T. hirsuta D7 produced only laccase (Lac), with the highest activity was 22.6 U/L on the 5th-day of the cultivation. At the same time, Ceriporia sp. BIOM 3 and C. dendriticum WM01 secreted both laccases (Lac) with the activities 0.2 U/L and 1.0 U/L, respectively, and manganese peroxidase (MnP) with the activities 0.1 U/L and 1.0 U/L, respectively. Among the fungi, T. hirsuta D7 efficiently degraded 65% Remazol Brilliant Blue–R (RBBR) dye within 72 h using the only laccase. This study shows that laccase may have a major role in synthetic dyes' decolorization process, followed by MnP and LiP.
愈创木酚存在下热带白腐真菌及其酶对雷马唑亮蓝- r染料的生物脱色
研究了热带白腐真菌及其酶对合成染料的脱色能力。在静态培养条件下,观察了三种新分离的真菌,即粗毛Trametes D7、Ceriporia sp.BIM3和树枝状环皮霉WM01产生木质素修饰酶(LMEs)9天。结果表明,在存在愈创木酚的情况下,LMEs的产量增加。T.hirsuta D7只产漆酶(Lac),培养第5天的活性最高,为22.6U/L。同时,Ceriporia sp.BIM3和C.dendrictum WM01同时分泌活性分别为0.2U/L和1.0U/L的漆酶(Lac)和活性分别为0.1U/L和1.0 U/L的锰过氧化物酶(MnP)。在真菌中,T.hirsuta D7仅用漆酶就可在72小时内有效降解65%的Remazol Brilliant Blue–R(RBBR)染料。本研究表明漆酶在合成染料脱色过程中可能起主要作用,其次是MnP和LiP。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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