Chemical Composition, Antioxidant, Antimicrobial, and Immunomodulatory Activity of Mandragora autumnalis Grown in Jordan

IF 0.7 Q4 CHEMISTRY, MEDICINAL

Natural Products Journal Pub Date : 2022-06-02 DOI:10.2174/2210315512666220602092915

A. Mahmod, Wamidh H. Talib

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引用次数: 0

Abstract

The activity of phytochemicals has a huge impact on pharmaceutical research and on the process of developing new drugs. Hence, numerous studies have been conducted to investigate the efficacy of different natural products and their potential to be used in different types of therapy. This study was carried out to investigate the biological activity of M. autumnalis crude extract and fractions, and the assessment was included antimicrobial activity, radical scavenging potential, and their effect on the immune system. The work also emphasizes determining the chemical constituents and the main bioactive compounds in M. autumnalis leaves. Ethanol crude extract was prepared by maceration method, followed by solvents fractionation using n-hexane, aqueous/methanol, and water. Phytochemical analysis of ethanol extract using LC-MS was conducted as well as total phenolic and flavonoids content. The immunomodulatory activity was also evaluated by measuring the effect of M. autumnalis on splenocytes proliferation in the presence and absence of mitogens. Nitro blue tetrazolium assay and neutral red method have been used to determine the effect of M. autumnalis on the phagocytic activity of macrophages and the function of pinocytosis, respectively. Antibacterial assay was applied using microtiter plate dilution method, and DPPH assay was used to determine the antioxidant activity. The phytochemical analysis has shown the presence of flavonoids, polyphenolic compounds, and other organic acids like quinic and chlorogenic acids. M. autumnalis aqueous fraction exhibited high antioxidant activity with IC₅₀ value of 23.67±0.3 μg/ml. Ethanol extract and n-hexane fraction inhibited the bacterial growth of P. auriginosa with MIC value of 25 mg/ml, while n-hexane fraction was more active against C. albicans with MIC value of 12.5 mg/ml. On the other hand, the immune assay revealed a slight modulation of the lymphocyte and macrophages activity compared to the control results. The results suggested that M. autumnalis leaves are rich in polyphenolic compounds and its crude extract and fractions exhibited antimicrobial and antioxidant potential. However, further research is needed to understand the effect of M. autumnalis on the immune parameters.

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约旦秋Mandragora autumnalis的化学成分、抗氧化、抗菌和免疫调节活性

植物化学物质的活性对药物研究和新药开发过程有着巨大的影响。因此，已经进行了大量研究来研究不同天然产物的疗效及其在不同类型治疗中的应用潜力。本研究旨在研究秋刀豆粗提取物和部分的生物活性，包括抗菌活性、清除自由基的潜力及其对免疫系统的影响。本工作还重点测定了秋葵叶片的化学成分和主要生物活性化合物。乙醇粗提取物通过浸渍法制备，然后使用正己烷、水/甲醇和水进行溶剂分馏。采用LC-MS对乙醇提取物进行了植物化学分析，并测定了总酚和黄酮含量。免疫调节活性也通过在有丝分裂原和无丝分裂原的情况下测量秋葵对脾细胞增殖的影响来评估。采用硝基蓝-四氮唑分析法和中性红法分别测定了秋葵对巨噬细胞吞噬活性和胞饮功能的影响。采用微量滴定板稀释法进行抗菌试验，DPPH法测定抗氧化活性。植物化学分析显示存在黄酮类化合物、多酚类化合物和其他有机酸，如奎宁酸和绿原酸。秋刀豆水性组分具有较高的IC抗氧化活性₅₀ 值为23.67±0.3μg/ml。乙醇提取物和正己烷组分对金黄色葡萄球菌的细菌生长具有抑制作用，MIC值为25mg/ml，而正己烷组份对白色念珠菌的MIC值更高，为12.5mg/ml。另一方面，免疫测定显示，与对照结果相比，淋巴细胞和巨噬细胞的活性略有调节。结果表明，秋葵叶片富含多酚类化合物，其粗提物和组分具有抗菌和抗氧化的潜力。然而，还需要进一步的研究来了解秋葵对免疫参数的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Products Journal CHEMISTRY, MEDICINAL-

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The Natural Products Journal a peer reviewed journal, aims to publish all the latest and outstanding developments in natural products. The Natural Products Journal publishes original research articles, full-length/mini reviews, letters and guest edited issues on all aspects of research and development in the field including: isolation, purification, structure elucidation, synthesis and bioactivity of chemical compounds found in nature.