Nicotiana cavicola as a host for production of recombinant proteins by Agrobacterium-mediated transient gene expression

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2019-10-27 DOI:10.7124/bc.000a11

Y. Sindarovska, Z. Olevinskaya, O. A. Demchenko, N. Y. Spivak, N. Kuchuk

引用次数: 1

Abstract

Aim. To analyze a novel plant species as a host for obtaining recombinant proteins via transient gene expression. Methods. Agrobacterium -mediated transient gene expression, protein analysis, statistical data processing. Results. N. cavicola plants demonstrate good biotechnological characteristics; they are susceptible to agrobacterial infection and plant viruses. Green fluorescent protein (GFP) and human interferon alpha were produced in N. cavicola after transient gene expression using two different vector systems. The level of recombinant proteins de-pended on the gene and the system used. GFP content reached 6.0 % and 12.6 % TSP (0.44 mg/g FW). The interferon antiviral activity of the leaf extracts was 840 IU/g FW and 1710 IU/g FW. Conclusion. Here we propose N. cavicola species as a novel host for obtaining recombinant proteins which can be used as an alternative to the N. benthamiana host.

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农杆菌介导的瞬时基因表达以烟叶为宿主生产重组蛋白

目标分析一种新的植物物种作为宿主，通过瞬时基因表达获得重组蛋白。方法。农杆菌介导的瞬时基因表达，蛋白质分析，统计数据处理。后果N.cavicola植物表现出良好的生物技术特性；它们易受农业细菌感染和植物病毒感染。绿色荧光蛋白（GFP）和人干扰素α在两种不同的载体系统瞬时表达后在豚鼠中产生。重组蛋白的水平取决于所使用的基因和系统。GFP含量达到6.0%，TSP含量达到12.6%（0.44mg/g FW）。叶提取物的干扰素抗病毒活性为840IU/g FW和1710IU/g FW。结论在这里，我们提出了N.cavicola物种作为一种新的宿主来获得重组蛋白，该重组蛋白可作为N.benthamiana宿主的替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.