{"title":"Measurement of Metabolic and Inflammatory Serum Markers and Immune Marker Gene Expression during Superovulation in Beef Cattle","authors":"A. Snider, D. Mclean, A. Menino","doi":"10.4236/OJAS.2021.112014","DOIUrl":null,"url":null,"abstract":"Health status of donor cows during superovulation is important to ensure \noptimal embryo quality at time of collection. Because nutritional and metabolic \nstatus impact embryo quality some form of nutritional supplementation is often \nprovided before and during superovulation. OmniGen-AF® (OG) feeding has \nbeen shown to assist in the maintenance of animal health through regulation of \nmetabolic status and balance and supporting aspects of immune function. We \nobserved feeding donor cows OG decreased percent degenerate embryos recovered \nfollowing superovulation increased serum progesterone concentration and \nimproved in vitro embryo development. Evaluation of OG feeding on markers of \nmetabolic function and inflammatory and immune function in beef cattle embryo \ndonors are reported here. Similarly, cow metabolic and inflammatory response \nwith repeated superovulation protocols is not known. Biomarkers to monitor and \nevaluate cow health during superovulation may provide management options to \nimprove embryo recovery and quality. Twenty-four Angus cross-bred cattle were \nrandomly assigned to four treatment groups, fed 0 or 56 g/hd/day for 49 days \nand superovulated with 200 or 400 mg Folltropin V (FSH). Blood was collected \nweekly for analyses. The protocol was repeated on all cows 90 - 120 d later with cows reassigned to their original \ngroups. No differences (P > 0.10) were observed due to OG feeding or \nFSH dose on metabolic and inflammatory markers. Replicate exerted a significant \neffect where serum concentration of albumin, IL1β, IL6, PGE2 and \nleptin were lower (P < 0.05) in \nReplicate 1 compared to 2. There was also a similar pattern of change in \nseveral of the metabolic and inflammatory markers during the superovulation \nprotocol where concentrations were higher at the time of estrus and ovulation. \nTaken together, physiologic changes during the estrous cycle and the number of \nsuperovulation protocols can modulate metabolic markers and inflammatory \nresponse.","PeriodicalId":62784,"journal":{"name":"动物科学期刊(英文)","volume":"11 1","pages":"175-196"},"PeriodicalIF":0.0000,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"动物科学期刊(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.4236/OJAS.2021.112014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Health status of donor cows during superovulation is important to ensure
optimal embryo quality at time of collection. Because nutritional and metabolic
status impact embryo quality some form of nutritional supplementation is often
provided before and during superovulation. OmniGen-AF® (OG) feeding has
been shown to assist in the maintenance of animal health through regulation of
metabolic status and balance and supporting aspects of immune function. We
observed feeding donor cows OG decreased percent degenerate embryos recovered
following superovulation increased serum progesterone concentration and
improved in vitro embryo development. Evaluation of OG feeding on markers of
metabolic function and inflammatory and immune function in beef cattle embryo
donors are reported here. Similarly, cow metabolic and inflammatory response
with repeated superovulation protocols is not known. Biomarkers to monitor and
evaluate cow health during superovulation may provide management options to
improve embryo recovery and quality. Twenty-four Angus cross-bred cattle were
randomly assigned to four treatment groups, fed 0 or 56 g/hd/day for 49 days
and superovulated with 200 or 400 mg Folltropin V (FSH). Blood was collected
weekly for analyses. The protocol was repeated on all cows 90 - 120 d later with cows reassigned to their original
groups. No differences (P > 0.10) were observed due to OG feeding or
FSH dose on metabolic and inflammatory markers. Replicate exerted a significant
effect where serum concentration of albumin, IL1β, IL6, PGE2 and
leptin were lower (P < 0.05) in
Replicate 1 compared to 2. There was also a similar pattern of change in
several of the metabolic and inflammatory markers during the superovulation
protocol where concentrations were higher at the time of estrus and ovulation.
Taken together, physiologic changes during the estrous cycle and the number of
superovulation protocols can modulate metabolic markers and inflammatory
response.