Issue Information TOC

Abstract

Cover: In Klimas et al. (https://doi.org/10.1002/cpcy.67), Representative results from sagittal sections of mouse striatum. Pre-expanded samples were stained with DAPI (blue) and labeled for tyrosine hydroxylase (green), synaptophysin (red), and α-internexin (magenta). Pre-expansion samples are shown on the left; post-expansion on the right. (A,B) Successful completion resulted in a 5.27-fold expansion of the tissue from A to B. (C,D) Magnified images of outlined regions in A and B, respectively. (E,F) A separate sample that was over-homogenized shows distortion and loss of fluorescent signals for tyrosine hydroxylase and synaptophysin. All images were taken on a spinning-disk confocal microscope using a 1.1-NA 40× (A-D) or 0.95-NA 20× (E,F) water-immersion objective. Scale bars: 10 µm (A,B; post-expansion physical size 52.7 µm); 5 µm (C,D; post-expansion physical size 26.4 µm); (E,F) 100 µm.