Autotetraploid plant production in endemic Onobrychis elata with colchicine treatments

IF 1.2 4区生物学 Q2 Agricultural and Biological Sciences

Journal of Applied Botany and Food Quality-Angewandte Botanik Pub Date : 2019-04-11 DOI:10.5073/JABFQ.2019.092.012

S. Avcı, M. Tuna, M. Kaya

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引用次数: 1

Abstract

This study aimed to induce autotetraploidy in endemic Onobrychis elata plants by colchicine treatment of seeds or seedlings. Colchicine was applied to O. elata directly on germinated seeds, pre-germinated seeds (root length of 3-8 mm), and apical regions (using cotton) under in vivo conditions. Out of a total of 1,210 colchicine-treated seeds that were evaluated, only 203 survived. There was an inverse relationship between the number of surviving plants and colchicine concentration and exposure time. The highest percentage of tetraploidy in surviving plants (50%) was obtained by applying 0.2% colchicine for 6 hours to pre-germinated seeds. No significant tetraploidy was achieved by colchicine application to seedlings. Flow cytometry observations indicated that DNA content varied between 0.99 and 1.06 pg in diploid plants (controls), while DNA content varied between 2.22 and 2.48 pg in tetraploid plants. It was concluded that tetraploid plants were induced successfully only in seedlings obtained from pre-germinated seeds, with their ploidy level confirmed via flow cytometry analysis.

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秋水仙碱处理对地方Onobrychis elata同源四倍体植株的生产

本研究旨在利用秋水仙碱处理的种子或幼苗，诱导地方性松藓属植物的同源四倍体。在体内条件下，将秋水仙碱直接施用于红花发芽种子、预发芽种子(根长3 ~ 8mm)和根尖区域(用棉花)。在评估的1,210个秋水仙碱处理过的种子中，只有203个存活下来。秋水仙碱浓度和暴露时间与成活株数呈反比关系。在预发芽种子上施用0.2%秋水仙碱6小时后，存活植株四倍体率最高(50%)。秋水仙碱对幼苗未产生显著的四倍性。流式细胞术观察显示，二倍体(对照)的DNA含量在0.99 ~ 1.06 pg之间，四倍体(对照)的DNA含量在2.22 ~ 2.48 pg之间。结果表明，四倍体植株只有在种子发芽后才能成功诱导，其倍性水平通过流式细胞术分析得到证实。

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来源期刊

Journal of Applied Botany and Food Quality-Angewandte Botanik 生物-植物科学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.