Identifying biofilm-forming strains of Staphylococcus epidermidis isolated from intravascular-catheterized patients by icaA and icaD genes

Q3 Health Professions
G. El-Sawaf, Sara M.A.I. Adlan, D. Metwally, M. M. Abo-Ollo, Medhat Mohammed Anwar Hamed, M. S. Abdel-Latif
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引用次数: 0

Abstract

Abstract Staphylococcus epidermidis has gained a substantial importance in recent years because it is one of the main causative agents of nosocomial infections. It requires a predisposed host in order to switch from a normal inhabitant of the human skin to a pathogenic flora. This study aimed to use icaA/icaD genes as biomarkers in differentiating biofilm-forming S. epidermidis, obtained from patients with intravascular catheter (IVC) infections, from other saprophytic strains. Twenty isolates of S. epidermidis obtained from 100 cases of intravascular catheter infections were investigated for the presence of the intracellular adhesion icaA and icaD genes by polymerase chain reaction (PCR) and for phenotypic biofilm production by qualitative Congo red agar assay (CRA). Results: Nine (~45%) S. epidermidis isolates out of 20 isolates collected from IVC infections were positive for both CRA (produce black colonies) and icaA/icaD genes; while 11 (~55%) S. epidermidis isolates were negative for CRA and icaA/icaD genes. Detection of icaA/icaD gene is a reliable, efficient, and more rapid tool for characterizing biofilm-forming strains of S. epidermidis. Keywords: Staphylococcus epidermidis; icaA; icaD; biofilm
应用icaA和icaD基因鉴定血管内导管患者表皮葡萄球菌生物膜形成菌株
表皮葡萄球菌是近年来引起医院感染的主要病原体之一,因此受到了广泛的关注。它需要一个预设的宿主,以便从人类皮肤的正常居民切换到致病菌群。本研究旨在利用icaA/icaD基因作为生物标志物,区分从血管内导管(IVC)感染患者中获得的形成生物膜的表皮葡萄球菌与其他腐生菌株。采用聚合酶链式反应(PCR)和刚果红琼脂质化试验(CRA)对从100例血管内导管感染中分离的20株表皮葡萄球菌细胞内粘附icaA和icaD基因的表达进行了研究。结果:20株IVC感染分离株中,9株(约45%)表皮葡萄球菌CRA(产生黑色菌落)和icaA/icaD基因均阳性;11株(~55%)表皮葡萄球菌CRA和icaA/icaD基因阴性。icaA/icaD基因检测是表征表皮葡萄球菌生物膜形成菌株的一种可靠、高效、快速的工具。关键词:表皮葡萄球菌;icaA;icaD;生物膜
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来源期刊
Chiang Mai University journal of natural sciences
Chiang Mai University journal of natural sciences Health Professions-Health Professions (miscellaneous)
CiteScore
1.70
自引率
0.00%
发文量
67
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