Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES

Pakistan Veterinary Journal Pub Date : 2020-04-01 DOI:10.29261/pakvetj/2019.109

Hammad Qamar

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引用次数: 10

Abstract

Received: Revised: Accepted: Published online: July 28, 2019 October 12, 2019 October 13, 2019 October 21, 2019 Among avian metabolic disorders, Tibial dyschondroplasia (TD) has a wide prevalence worldwide in chickens that are identified by non-mineralization and avascularization of the growth plate (GP). Grape seed extract (GSE) reduces the inflammation and oxidative stress by scavenging free oxygen specie and has some anti-cancer and angiogenic activity. This study was carried out to evaluate the role of GSE for the therapy of the chickens affected with TD. Arbor Acres broiler chicks (n=240) weighing 47±6g were fed starter feed till first 3 days and then portioned equally into three groups (Control, TD and GSE group; n=80 each). Thiram was incorporated in the feed of TD and GSE chicks until day 6 for inducing TD (50 mg/ kg of feed), whereas usual feed was offered to control group. All the birds in TD and GSE group showed the symptoms of TD on day 7 and GSE group was given GSE (50 mg/kg/day) intraperitoneally till the termination of the experiment. Chickens fed with thiram-feed exhibited high TD score and GP measurement. After the GSE treatment, the TD score dropped remarkably and, inflammation and TD lesions reduced. Moreover, body weight gain and liver weight were increased in comparison with TD chicks after application of GSE. GSE supplementation alleviated the adverse effects of thiram and increase in angiogenesis was observed. In conclusion, GSE recovered the TD affected broilers through downregulating the HIF-1α, Hsp90 and CA2 expressions. ©2019 PVJ. All rights reserved

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葡萄籽提取物对鸡胫骨软骨发育不良发生率、肝脏重量和胫骨血管生成的影响

2019年7月28日2019年10月12日2019年10月13日2019年10月21日2019年10月21日在禽类代谢疾病中，胫骨软骨发育不良(Tibial dyschondroplasia, TD)在全球范围内广泛流行，以生长板(growth plate, GP)的非矿化和无血管化为特征。葡萄籽提取物(GSE)通过清除游离氧来减轻炎症和氧化应激，并具有一定的抗癌和血管生成活性。本研究旨在评价GSE对鸡TD的治疗作用。选取240只体重为47±6g的爱拔益加肉仔鸡，饲喂初饲料至前3 d，然后随机分为3组(对照组、TD组和GSE组;n = 80)。在TD和GSE雏鸡的饲料中添加Thiram (50 mg/ kg饲料)，直至第6天诱导TD，对照组饲喂普通饲料。试验第7天，TD组和GSE组均出现TD症状，GSE组给予GSE (50 mg/kg/d)腹腔注射至试验结束。饲粮中添加三聚氰胺的鸡具有较高的TD评分和GP测量值。经GSE治疗后，TD评分明显下降，炎症和TD病变减少。此外，与TD雏鸡相比，GSE的增重和肝重均有所提高。GSE的补充减轻了thiram的不良反应，并增加了血管生成。综上所述，GSE通过下调HIF-1α、Hsp90和CA2的表达来恢复TD病肉鸡。©2019 PVJ。版权所有

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.