Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art

IF 2.35

BMC Chemical Engineering Pub Date : 2019-06-05 DOI:10.1186/s42480-019-0013-9

Alok Patel, Io Antonopoulou, Josefine Enman, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas

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引用次数: 33

Abstract

Oleaginous microorganisms are among the most promising feedstocks for the production of lipids for biofuels and oleochemicals. Lipids are synthesized in intracellular compartments in the form of lipid droplets. Therefore, their qualitative and quantitative analysis requires an initial pretreatment step that allows their extraction. Lipid extraction techniques vary with the type of microorganism but, in general, the presence of an outer membrane or cell wall limits their recovery. This review discusses the various types of oleaginous microorganisms, their lipid accumulating capabilities, lipid extraction techniques, and the pretreatment of cellular biomass for enhanced lipid recovery. Conventional methods for lipid quantification include gravimetric and chromatographic approaches; whereas non-conventional methods are based on infrared, Raman, nuclear magnetic resonance, and fluorescence spectroscopic analysis. Recent advances in these methods, their limitations, and fields of application are discussed, with the aim of providing a guide for selecting the best method or combination of methods for lipid quantification.

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产油微生物中的脂质检测和定量:当前技术状况的概述

产油微生物是生产生物燃料和油脂化学品用脂类最有前途的原料之一。脂质在细胞内以脂滴的形式合成。因此，它们的定性和定量分析需要一个初始的预处理步骤，以便提取它们。脂质提取技术随微生物的类型而变化，但一般来说，外膜或细胞壁的存在限制了它们的回收。本文综述了各种类型的产油微生物，它们的脂质积累能力，脂质提取技术，以及提高脂质回收的细胞生物质预处理。常规的脂质定量方法包括重量法和色谱法;而非传统的方法是基于红外、拉曼、核磁共振和荧光光谱分析。本文讨论了这些方法的最新进展、局限性和应用领域，旨在为选择最佳的脂质定量方法或方法组合提供指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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BMC Chemical Engineering

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