Association of platelet glycoprotein IIIa polymorphism as a risk factor for acute ischemic stroke in Egyptian patients

Abstract

Context Association of genetic variants of platelet receptors and their inferences on cerebral stroke is a major concern. Aims The current study evaluates the genetic polymorphism of platelet GPIIIa as a risk factor in Egyptian patients with ischemic cerebrovascular stroke. Settings and design A total of 50 patients with ischemic stroke were recruited from the Neurology Department, in addition to 50 control individuals matching the study group in age and sex. Patients and methods Data were collected using an interview questionnaire, clinical and neurological examination, and laboratory assessment, which included hematological assessment, biochemical assessment, and molecular assessment of genotyping of GPIIIa polymorphism by PCR-RFLP technique using endonuclease restriction alongside Msp-I enzyme. Statistical analysis To compare control and study groups, independent t test in parametric data and Mann–Whitney for nonparametric data were used. Results Regarding GPIIIa (PlA2/A2) genotypic distribution of the studied groups, there was a statistically significant difference between patients with ischemic cerebrovascular stroke and controls. Dyslipidemia and platelet GPIIIa genotype showed the highest odds ratio. On binary regression analysis, the role of the platelet genotype as a risk factor of stroke development alone is maximized. In the copresence of other risk factors, its role is minimized. Conclusions The GPIIIa (PlA1/PlA2) polymorphism is a highly predictive and reliable biomarker for ischemic cerebrovascular stroke.