Species Identification and Unlocking Hidden Genetic Diversity of Confiscated Slow Lorises (Nycticebus spp.) Based on Mitochondrial DNA Markers

IF 1.2 4区生物学 Q2 ZOOLOGY

Folia Primatologica Pub Date : 2019-10-08 DOI:10.1159/000500007

C. Kongrit, Darunee Markviriya, Phadet Laithong, J. Khudamrongsawat

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引用次数: 5

Abstract

Confiscated slow lorises (Nycticebus spp.) at Bangpra Water-Bird Breeding Center (BWBC) in Thailand provided an opportunity to demonstrate the application of noninvasive genetic approaches for species identification when morphology of the animals was ambiguous. The slow lorises at BWBC had been assigned to either N. bengalensis or N. pygmaeus, based on body size. However, the morphology of N. bengalensis is highly variable and overlaps with that of N. coucang (sensu stricto). Phylogenetic analysis of cytochrome b and d-loop mitochondrial regions placed all confiscated N. pygmaeus with the published sequences of N. pygmaeus and distinguished them from other Nycticebus. All other confiscated individuals formed a monophyletic clade, most individuals grouping with published N. bengalensis sequences from wild populations in Vietnam and distinct from Peninsular Malaysian and Sumatran N. coucang, Javan N. javanicus and Bornean N. menagensis. Six individuals within the N. bengalensis clade formed a separate subgroup that did not group with any reference material as indicated by phylogenetic and haplotype network analyses. Whether these trafficked individuals are undiscovered wild populations will require further investigation. Additional genetic studies of wild slow loris populations in different regions are therefore urgently required for reference to aid the protection and conservation of these threatened species.

查看原文本刊更多论文

基于线粒体DNA标记的慢Lorises（Nycticebus spp.）物种鉴定及隐性遗传多样性的揭示

泰国Bangpra水鸟繁殖中心（BWBC）没收的懒猴（Nycticebus spp.）提供了一个机会，在动物形态不明确的情况下，展示了非侵入性遗传方法在物种识别中的应用。根据体型，BWBC的懒猴被分为孟加拉懒猴或俾格麦懒猴。然而，孟加拉猪笼草的形态变化很大，与库藏猪笼草（狭义）的形态重叠。细胞色素b和d环线粒体区域的系统发育分析将所有没收的毕格曼猪笼草与已发表的毕格门猪笼草序列放在一起，并将其与其他毕格曼牛区分开来。所有其他被没收的个体都形成了一个单系分支，大多数个体与越南野生种群中已发表的孟加拉猪笼草序列分组，与马来西亚半岛和苏门答腊猪笼草、爪哇猪笼草和婆罗洲猪笼草不同。孟加拉猪笼草分支中的六个个体形成了一个单独的亚群，如系统发育和单倍型网络分析所示，该亚群没有与任何参考物质分组。这些被贩卖的个体是否是未被发现的野生种群，还需要进一步调查。因此，迫切需要对不同地区的野生懒猴种群进行额外的遗传研究，以供参考，以帮助保护和养护这些受威胁的物种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Primatologica 生物-动物学

CiteScore

3.30

自引率

10.50%

发文量

审稿时长

>12 weeks

期刊介绍： Recognizing that research in human biology must be founded on a comparative knowledge of our closest relatives, this journal is the natural scientist''s ideal means of access to the best of current primate research. ''Folia Primatologica'' covers fields as diverse as molecular biology and social behaviour, and features articles on ecology, conservation, palaeontology, systematics and functional anatomy. In-depth articles and invited reviews are contributed by the world’s leading primatologists. In addition, special issues provide rapid peer-reviewed publication of conference proceedings. ''Folia Primatologica'' is one of the top-rated primatology publications and is acknowledged worldwide as a high-impact core journal for primatologists, zoologists and anthropologists.