Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

IF 0.8 4区工程技术 Q4 ENGINEERING, CHEMICAL

Hemijska Industrija Pub Date : 2017-09-25 DOI:10.2298/HEMIND160421046M

G. M. Miljkovic, Z. Davidovic, S. Kralj, S. Šiler-Marinković, D. M. Rajilic-Stojanovic, I. S. Dimitrijević-Branković

{"title":"Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate","authors":"G. M. Miljkovic, Z. Davidovic, S. Kralj, S. Šiler-Marinković, D. M. Rajilic-Stojanovic, I. S. Dimitrijević-Branković","doi":"10.2298/HEMIND160421046M","DOIUrl":null,"url":null,"abstract":"The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 °C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K 2 HPO 4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 °C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca 2+ , Mg 2+ , Fe 2+ and Co 2+ led to a minor increase of DS activity, while the addition of Mn 2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.","PeriodicalId":12913,"journal":{"name":"Hemijska Industrija","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2017-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hemijska Industrija","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.2298/HEMIND160421046M","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 7

Abstract

The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 °C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K 2 HPO 4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 °C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca 2+ , Mg 2+ , Fe 2+ and Co 2+ led to a minor increase of DS activity, while the addition of Mn 2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.

查看原文本刊更多论文

水开菲尔颗粒分离物肠系膜Leuconostoc mesenteroides T3葡聚糖蔗糖酶的鉴定

研究并优化了从水开菲尔粒中分离得到的间质明串珠菌T3生产右旋糖酐蔗糖酶（DS）的工艺条件。当培养物在23°C下生长并在静态培养条件下使用经典的Tsuchiya培养基生产DS时，细菌上清液的活性达到3.1U/ml。与对照相比，蔗糖浓度增加到7%导致DS活性增加52%。含有2%牛肉提取物和1%酵母提取物的培养基产生4.52 U/ml，比对照（含有2%酵母提取物）高47%。最后，K2 HPO4浓度从2%增加到3%，酶活性增加了2 8%。通过聚乙二醇400分级纯化的酶在30°C和pH 5.4下显示出最大活性。Zymogram分析证实了约180kDa的DS的存在。二价阳离子Ca2+、Mg2+、Fe2+和Co2+的加入使DS活性略有增加，而Mn2+的添加最为显著，增加了73%。这些发现将来自间质明串珠菌T3的右旋糖酐蔗糖酶归类为生产右旋糖聚糖的有前途的候选者，其在各种工业中具有许多应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Hemijska Industrija 工程技术-工程：化工

CiteScore

1.60

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal Hemijska industrija (abbreviation Hem. Ind.) is publishing papers in the field of Chemical Engineering (Transport phenomena; Process Modeling, Simulation and Optimization; Thermodynamics; Separation Processes; Reactor Engineering; Electrochemical Engineering; Petrochemical Engineering), Biochemical Engineering (Bioreactors; Protein Engineering; Kinetics of Bioprocesses), Engineering of Materials (Polymers; Metal materials; Non-metal materials; Biomaterials), Environmental Engineeringand Applied Chemistry. The journal is published bimonthly by the Association of Chemical Engineers of Serbia (a member of EFCE - European Federation of Chemical Engineering). In addition to professional articles of importance to industry, scientific research papers are published, not only from our country but from all over the world. It also contains topics such as business news, science and technology news, information on new apparatus and equipment, and articles on environmental protection.