Season- and sex-related variation in mucin secretions of the striped Venus clam, Chamelea gallina (Linnaeus, 1758) (Bivalvia: Veneridae)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-29 DOI:10.1080/24750263.2023.2190343

M. V. Guglielmi, D. Semeraro, D. Mentino, M. Mastrodonato, F. Mastrototaro, G. Scillitani

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引用次数: 1

Abstract

Abstract An in situ analysis of mucin secretions along the annual cycle was performed on the striped Venus clam, Chamelea gallina. Individuals of both sexes from an exploited stock of Margherita di Savoia (Southern Adriatic Sea, Central Mediterranean Sea) were made in June 2020 and 2021, and in January 2021 and 2022, representative of summer and winter seasons, respectively. Tissues from the foot and the gills were analyzed for histochemical and lectin-histochemical analyses. Staining with Periodic Acid-Schiff, Alcian Blue pH 2.5 and High-Iron-Diamine indicated that mucins from both the foot and the gills were acidic, mostly sulfated. Lectin-binding analyses with PNA, SBA, WGA, LTA, UEA-I, AAA, SNA, MAA-II and ConA indicated the presence of N-acetyl-glycosaminylated, mannosylated and fucosylated residuals in the saccharidic chains. In the gills, the amount of acidic and glycosaminylated residuals was higher in summer in both sexes, whereas fucosylation was similar along the sampling period and mannosylation was never observed. In the foot, both surface epithelial and subepidermal secreting cells increased sulfation in winter in males, but not in females. Glycosaminylation and fucosylation were observed only in the surface cells of males. Mannosylated residuals were observed in all the foot cell types in both sexes. It is hypothesized that the observed qualitative and quantitative variations in mucin secretion is linked to the reproductive cycle. Highlights The mucocytes present in the gill filaments of the clam produce an acid mucus, with sulfate residues and N-acetylglucosamine and fucose chains. In the foot, the subepithelial mucocytes produce an acidic, sulfate secretion with mannosylated residuals. The foot epithelium shows an acidic, sulfated mucus composition with N-acetylglucosamine, fucose and mannose chains. The results show that males have greater variability in mucus composition. The observed variation could be related to different energy allocation between sexes in the development of gametes.

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条纹维纳斯蛤黏蛋白分泌的季节和性别变化(林奈，1758)(双壳目:蛤科)

摘要对带条纹的金花蛤（Chamelea gallina）的粘蛋白分泌物进行了年周期的原位分析。2020年6月和2021年6月，以及2021年1月和2022年1月，分别代表夏季和冬季，从被开采的萨沃亚玛格丽塔种群（亚得里亚海南部、地中海中部）中采集了两性个体。对足和鳃的组织进行组织化学和凝集素组织化学分析。用碘酸Schiff、Alcian Blue pH 2.5和高铁二胺染色表明，来自脚和鳃的粘蛋白都是酸性的，大部分是硫酸化的。用PNA、SBA、WGA、LTA、UEA-I、AAA、SNA、MAA-II和ConA进行的凝集素结合分析表明，糖精链中存在N-乙酰基-氨基甘糖基化、甘露糖基化和岩藻糖基化的残基。在鳃中，夏季两性的酸性和糖胺基化残留物的量都较高，而岩藻糖基化在采样期间相似，从未观察到甘露糖基化。在足部，雄性的表面上皮和表皮下分泌细胞在冬季都增加了硫酸化，但雌性没有。糖氨基化和岩藻糖基化仅在雄性的表面细胞中观察到。在两性的所有足细胞类型中都观察到甘露糖基化残留物。据推测，观察到的粘蛋白分泌的定性和定量变化与生殖周期有关。亮点存在于蛤蜊鳃丝中的粘液细胞产生酸性粘液，带有硫酸盐残基、N-乙酰葡糖胺和岩藻糖链。在足部，上皮下粘膜细胞产生酸性硫酸盐分泌物，并伴有甘露糖基化残留物。足部上皮显示酸性硫酸粘液成分，含有N-乙酰葡糖胺、岩藻糖和甘露糖链。研究结果表明，雄性的粘液成分变化较大。观察到的变异可能与配子发育过程中两性能量分配的不同有关。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.