miR-191 inhibits oxygen-induced retinal neovascularization in mice

Q4 Medicine

中华眼底病杂志 Pub Date : 2019-09-25 DOI:10.3760/CMA.J.ISSN.1005-1015.2019.05.010

Boshi Liu, Lijie Dong, Xiaorong Li, Yan Zhang, Mingliang Zhang, Xun Liu, Liangyu Huang, Mianmian Wu, Manhong Xu, Ruihong Su, Zhe Zhang

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引用次数: 1

Abstract

Objective To observe the inhibitory effect of lentiviral vector miR-191 (LV-191) on retinal neovascularization (RNV) in mice model of oxygen-induced retinopathy (OIR). Methods Eighty healthy 7-day-old C57BL/6J mice were randomly divided into 5 groups including normal group, non-intervention group, normal saline (NS) group, LV-191 group and LV-green fluorescent protein (GFP) group, 16 mice in each group. The OIR model was established in the non-intervention group, NS group, LV-191 group and LV-GFP group. NS group, LV-191 group and LV-GFP group were given an intravitreal injection of 1 μl of NS, LV-191 and LV-GFP at the age of 12 days. No injection was performed in the non-intervention group. In normal group,newborn mouse were maintained in room air form P0 to P17, and no treatment was performed. Mice in all five groups were euthanized at P17. Retinal neovasculation (RNV) was evaluated by counting the number of pre-retinal neovascular cells and analysis of non-perfusion area area by immunofluorescent staining of the mouse retina. Real-time quantitative PCR (RT-PCR) to detect miR -191 and P21 expression of retinal tissue. Results In the LV-191 group, the non-perfusion area were both significantly smaller than those in non-intervention group, NS group and LV-GFP group (F=127.20, P<0.001). The number of pre-retinal neovascular cell nuclei in retinas from LV-191 group were obviously lower than those in the retinas from non-intervention group, NS group and LV-GFP group (F=31.71, P<0.05). RT-PCR showed that the LV-191 and P21 level of LV-191 group increased significantly than other groups (F=10.95, 15.60; P<0.05). Conclusion Intravitreal injection of LV- 191 inhibits RNV in mice model of OIR possibly through up-regulating p21. Key words: Retinal neovascularization/prevention c Lentivirus infections; Animal experimentation

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miR-191抑制氧诱导的小鼠视网膜新生血管

目的观察慢病毒载体miR-191 (LV-191)对氧致视网膜病变(OIR)小鼠视网膜新生血管(RNV)的抑制作用。方法健康7日龄C57BL/6J小鼠80只，随机分为正常组、不干预组、生理盐水组、LV-191组和lv -绿色荧光蛋白组5组，每组16只。非干预组、NS组、LV-191组、LV-GFP组分别建立OIR模型。NS组、LV-191组和LV-GFP组大鼠在12日龄时玻璃体内注射NS、LV-191和LV-GFP各1 μl。非干预组不进行注射。正常组新生小鼠保持在P0 ~ P17的室内空气中，不进行任何处理。五组小鼠均在P17时安乐死。通过小鼠视网膜免疫荧光染色，计数视网膜前新生血管细胞数，分析非灌注区面积，评价视网膜新生血管(RNV)。实时荧光定量PCR (RT-PCR)检测miR -191和P21在视网膜组织中的表达。结果LV-191组大鼠非灌注面积均显著小于非干预组、NS组和LV-GFP组(F=127.20, P<0.001)。LV-191组视网膜前新生细胞核数量明显低于非干预组、NS组和LV-GFP组(F=31.71, P<0.05)。RT-PCR结果显示，LV-191组的LV-191和P21水平显著高于其他各组(F=10.95, 15.60;P < 0.05)。结论玻璃体内注射LV- 191可能通过上调p21抑制RNV。关键词:视网膜新生血管;预防;慢病毒感染;动物实验

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来源期刊

中华眼底病杂志 Medicine-Ophthalmology

CiteScore

0.40

自引率

0.00%

发文量

5383

期刊介绍： Chinese Journal of Ocular Fundus Diseases is the only scientific journal in my country that focuses on reporting fundus diseases. Its purpose is to combine clinical and basic research, and to give equal importance to improvement and popularization. It comprehensively reflects the leading clinical and basic research results of fundus disease disciplines in my country; cultivates professional talents in fundus disease, promotes the development of fundus disease disciplines in my country; and promotes academic exchanges on fundus disease at home and abroad. The coverage includes clinical and basic research results of posterior segment diseases such as retina, uveal tract, vitreous body, visual pathway, and internal eye diseases related to systemic diseases. The readers are medical workers and researchers related to clinical and basic research of fundus diseases. According to the journal retrieval report of the Chinese Institute of Scientific and Technological Information, the comprehensive ranking impact factor and total citation frequency of the Chinese Journal of Ocular Fundus Diseases have been among the best in the disciplines of ophthalmology, otolaryngology, and ophthalmology in my country for many years. The papers published have been included in many important databases at home and abroad, such as Scopus, Peking University Core, and China Science Citation Database (CSCD).