Expression of integrin α5 in cervical cancer and its significance

Q4 Medicine

肿瘤研究与临床 Pub Date : 2019-10-28 DOI:10.3760/CMA.J.ISSN.1006-9801.2019.10.003

Weimin Du, Yumin Zhang, Xueying Tan, Xiaona Xu, Ping Zhang

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Abstract

Objective To investigate the expression of integrin α5 in cervical cancer, and to explore its relationship with clinicopathological characteristics of patients with cervical cancer. Methods Immunohistochemistry was used to detect the expression of integrin α 5 in cervical cancer tissues of 60 cases and normal cervical paraffin-embeded tissues of 20 cases of benign uterine lesions undergoing hysterectomy from Qingdao Municipal Hospital between January 2014 and December 2017. Real-time quantitative polymerase chain reaction (RT-PCR) was used to detect the mRNA expression level of integrin α5 in 20 fresh cervical cancer tissues and 20 normal cervical tissues collected from benign cervical lesions in Qingdao Municipal Hospital between January 2018 and July 2018. The relationship between the expression of integrin α5 and the clinicopathological characteristics of patients with cervical cancer was analyzed. Results The positive expression rate of integrin α5 protein in cervical cancer and normal cervical tissues was 63.3% (38/60), 35.0% (7/20), respectively, and the difference was statistically significant (χ2 = 4.893, P < 0.05). The expression of integrin α5 mRNA in cervical cancer was 1.6±0.4 times as high as that in normal cervical tissues (t = 5.529, P < 0.01). The positive expression of integrin α5 protein was associated with lymph node metastasis in cervical cancer patients (Z = -2.636, P = 0.008). Conclusion The high expression of integrin α5 is related to lymph node metastasis of cervical cancer, and integrin α5 may be a new potential target for treatment of cervical cancer. Key words: Uterine cervical neoplasms; Integrin alpha 5; Lymph node metastasis

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整合素α5在癌症中的表达及其意义

目的探讨整合素α5在宫颈癌中的表达，并探讨其与宫颈癌患者临床病理特征的关系。方法采用免疫组织化学方法检测整合素α 5在青岛市属医院2014年1月至2017年12月间60例宫颈癌组织和20例良性子宫病变行子宫切除术的正常宫颈石蜡包埋组织中的表达。采用实时定量聚合酶链反应(RT-PCR)检测2018年1月至2018年7月青岛市市属医院采集的20例宫颈良性病变患者新鲜宫颈癌组织和20例正常宫颈组织中整合素α5 mRNA表达水平。分析整合素α5的表达与宫颈癌患者临床病理特征的关系。结果整合素α5蛋白在宫颈癌与正常宫颈组织中的阳性表达率分别为63.3%(38/60)、35.0%(7/20)，差异有统计学意义(χ2 = 4.893, P < 0.05)。整合素α5 mRNA在宫颈癌组织中的表达是宫颈正常组织的1.6±0.4倍(t = 5.529, P < 0.01)。整合素α5蛋白阳性表达与宫颈癌患者淋巴结转移相关(Z = -2.636, P = 0.008)。结论整合素α5的高表达与宫颈癌淋巴结转移有关，整合素α5可能是治疗宫颈癌的一个新的潜在靶点。关键词:子宫颈肿瘤;整合素α 5;淋巴结转移

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来源期刊

肿瘤研究与临床 Medicine-Oncology

CiteScore

0.10

自引率

0.00%

发文量

7737

期刊介绍： "Cancer Research and Clinic" is a series of magazines of the Chinese Medical Association under the supervision of the National Health Commission and sponsored by the Chinese Medical Association. It mainly reflects scientific research results and academic trends in the field of malignant tumors. The main columns include monographs, guidelines and consensus, standards and norms, treatises, short treatises, survey reports, reviews, clinical pathology (case) discussions, case reports, etc. The readers are middle- and senior-level medical staff engaged in basic research and clinical work on malignant tumors.