Experimental study on the expression of ubiquitin-specific protease-25 in temporal cortex of epileptic rats

Q4 Medicine

中华神经外科杂志 Pub Date : 2020-03-28 DOI:10.3760/CMA.J.CN112050-20191105-00476

Ye Chen, Yujiao Wang, Zhong-fang Shi, Xu Yan, Li-xin Xu, Li-ping Dong, Zhenrong Sun, F. Yuan

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引用次数: 0

Abstract

Objective To study the expression of ubiquitin-specific protease 25 (USP25) in the temporal cortex of the kainic acid (KA) induced epilepsy rat model. Methods Fifty-two male SD rats were randomly divided into the epilepsy model group (n=39) and sham-operated control group (n=13) with the random number table. The epilepsy model group was established by injecting KA into the amygdala, then the epileptic rats were randomly divided into 3 groups according to the modeling success time: 1 day for acute period, 7 days for latent period and 30 days for chronic period (13 rats in each group). Those rats were sampled at the end of observation. Rats in the control group were injected with normal saline into the amygdala and sampled together with those in the experimental group. Immunohistochemistry and immunofluorescence double labeling was used to test USP25 expression and its co-expression with neurons (NeuN) and astrocytes (GFAP). Quantitative real-time PCR and Western blot were used to assess the change of USP25 in the temporal cortex of rats. Results In the ipsilateral temporal cortex, the positive cells of co-expression of USP25 and NeuN were increased in the later stage of epilepsy in the epilepsy model group, and the expression levels of USP25 mRNA and protein in different stages of epilepsy varied significantly (F= 25.48 and 7.68 respectively, both P<0.05). Compared with the control group (mRNA level: 1.00±0.36, protein level: 1.00±0.46), the expression of USP25 in the latent group (mRNA level: 10.80±4.82, protein level: 1.88±0.32) and the chronic group (mRNA level: 12.97±4.48, protein level: 1.92±0.26) were increased significantly (all P<0.05). In the contralateral temporal cortex, the expression levels of USP25 mRNA and protein in different stages of epilepsy also varied significantly (F=86.86 and 6.65 respectively, both P<0.05). Conclusions The increased expression of USP25 in the temporal cortex after the latent period has suggested that the deubiquitination pathway is involved in the chronic pathological process of temporal lobe epilepsy. Key words: Epilepsy, temporal lobe; Ubiquitin-specific protease 25; Rats

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泛素特异性蛋白酶25在癫痫大鼠颞叶皮层表达的实验研究

目的研究泛素特异性蛋白酶25 (USP25)在kainic acid (KA)诱导的癫痫大鼠颞叶皮层的表达。方法52只雄性SD大鼠采用随机数字表法随机分为癫痫模型组(n=39)和假手术对照组(n=13)。癫痫模型组采用杏仁核注射KA建立，然后根据成功造模时间将癫痫大鼠随机分为急性期1 d、潜伏期7 d、慢性期30 d 3组，每组13只。这些大鼠在观察结束时取样。对照组大鼠与实验组大鼠一起在杏仁核内注射生理盐水取样。采用免疫组织化学和免疫荧光双标记法检测USP25的表达及其与神经元(NeuN)和星形胶质细胞(GFAP)的共表达。采用实时荧光定量PCR和Western blot检测大鼠颞叶皮层中USP25的表达变化。结果癫痫模型组同侧颞叶皮层USP25和NeuN共表达阳性细胞在癫痫晚期增多，且USP25 mRNA和蛋白在癫痫不同阶段的表达水平差异有统计学意义(F= 25.48、7.68,P均<0.05)。与对照组(mRNA水平:1.00±0.36，蛋白水平:1.00±0.46)相比，潜伏组(mRNA水平:10.80±4.82，蛋白水平:1.88±0.32)和慢性组(mRNA水平:12.97±4.48，蛋白水平:1.92±0.26)USP25的表达均显著升高(均P<0.05)。在对侧颞叶皮层，USP25 mRNA和蛋白在癫痫不同阶段的表达水平也有显著差异(F=86.86、6.65,P均<0.05)。结论潜伏期后颞叶皮层USP25表达升高，提示去泛素化通路参与了颞叶癫痫的慢性病理过程。关键词:癫痫;颞叶;泛素特异性蛋白酶25;老鼠

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来源期刊

中华神经外科杂志 Medicine-Surgery

CiteScore

0.10

自引率

0.00%

发文量

10706

期刊介绍： Chinese Journal of Neurosurgery is one of the series of journals organized by the Chinese Medical Association under the supervision of the China Association for Science and Technology. The journal is aimed at neurosurgeons and related researchers, and reports on the leading scientific research results and clinical experience in the field of neurosurgery, as well as the basic theoretical research closely related to neurosurgery.Chinese Journal of Neurosurgery has been included in many famous domestic search organizations, such as China Knowledge Resources Database, China Biomedical Journal Citation Database, Chinese Biomedical Journal Literature Database, China Science Citation Database, China Biomedical Literature Database, China Science and Technology Paper Citation Statistical Analysis Database, and China Science and Technology Journal Full Text Database, Wanfang Data Database of Medical Journals, etc.