Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys

IF 1.5 4区 农林科学 Q2 FORESTRY
Júlio Cézar Tannure Faria, Caius Ribeiro-Kumara, R. R. Costa, E. Nieri, D. de Carvalho, J. P. Pinto, A. R. S. Neto, G. E. Brondani
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引用次数: 4

Abstract

Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications of plant tissue culture. It can be used to clone specific plants with desired traits and reduce the cost of plant propagation. In this study, we developed a protocol for micropropagation of Eucalyptus microcorys F.Muell using a selected mature tree, in which we tested various combinations of different culture media and evaluated the use of biodegradable polyester-based microvessels during the adventitious rooting and acclimatisation phases. Methods: Epicormic shoots were used as an explant source. After the in vitro explant establishment and multiplication, we tested 8 combinations of BAP, NAA and IBA in the elongation phase. Three types of microvessels were tested in the adventitious rooting phase and acclimatisation of the microcuttings. Results: Epicormic shoots had an establishment percentage of 40.6% and a total of 820 explants were generated by the 11th subculture, with an average of 12 buds per explant. Best shoot elongation results were achieved with BAP (0.05 mg L-1) + NAA (1 mg L-1) and BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1) combinations, whereas microvessel types M2 and M3 provided higher rooting and acclimatisation. According to the results of ISSR markers, at the end of 535 days of in vitro cultivation, cloning was successful between acclimatised micro-plantlets and the parent plant. Conclusions: The micropropagation protocol using microvessels was efficient in producing E. microcorys clonal micro-plantlets and is recommended for further studies with this species, and for testing in the micropropagation of other species.
可生物降解聚酯微血管在桉树成熟微细胞微繁殖中的应用
背景:利用小繁殖体的离体无性繁殖技术是植物组织培养的主要应用之一。它可以用于克隆具有所需性状的特定植物,降低植物繁殖成本。在本研究中,我们利用一棵成熟的桉树开发了一种微桉(Eucalyptus microcorys F.Muell)的微繁方案,在该方案中,我们测试了不同培养基的各种组合,并评估了在不定根和驯化阶段使用可生物降解聚酯基微管的情况。方法:外植体为外植体。在离体外植体建立和增殖后,我们在伸长期测试了BAP、NAA和IBA的8种组合。在扦插不定根期和扦插驯化期对三种类型的微血管进行了试验。结果:外生芽成活率为40.6%,第11次传代共产生外植体820个,平均每个外植体产生12个芽。BAP (0.05 mg L-1) + NAA (1 mg L-1)和BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1)组合的芽伸长效果最好,而M2和M3微血管类型的生根和驯化效果更好。根据ISSR标记的结果,在离体培养535 d时,驯化后的微植株与亲本植株克隆成功。结论:微血管微繁技术可有效地培养出微球莲无性系植株,值得进一步研究,并可用于其他物种的微繁试验。
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来源期刊
CiteScore
2.20
自引率
13.30%
发文量
20
审稿时长
39 weeks
期刊介绍: The New Zealand Journal of Forestry Science is an international journal covering the breadth of forestry science. Planted forests are a particular focus but manuscripts on a wide range of forestry topics will also be considered. The journal''s scope covers forestry species, which are those capable of reaching at least five metres in height at maturity in the place they are located, but not grown or managed primarily for fruit or nut production.
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