PROLONGATION OF CLEANSING DAMAGED TISSUES FROM DETRITUS USING EXOSOMES OF MULTIPOTENT STROMAL CELLS

Q4 Medicine
I. Maiborodin, A. Shevela, S. Marchukov, V. Morozov, V. Matveeva, V. I. Maiborodina, A. M. Novikov, Y. Tornuev, B. Churin, A. Shevela
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引用次数: 0

Abstract

Objective. To study the effect of exosomes of multipotent mesenchymal stromal cells (EMSCs) on soft tissues damaged during implantation of a metal screw into the bone. Methods. A defect (2 mm in diameter and 4 mm in depth) was created in the tibial proximal condyles of outbred rabbits. Metal screws were implanted into the defect by preliminary injection of saline (control, n=9 animals) or 19.2 μg of EMSCs per limb (experiment, n=10 rabbits). After 3, 7 and 10 days following the operation, the animals were taken out from the experiment; histological sections of soft tissues from the condyle surface, stained by hematoxylin and eosin were studied using light microscopy. Results. The use of water cooling in the process of introducing the metal implant into the tibial proximal condyle does not lead to complete removal of small bone fragments, which are subsequently either eliminated outward with wound discharge, or are destroyed and are subjected to lysis by macrophages. As a result of the EMSC effect on soft tissues near the site of damage, the activity of the postoperative inflammation reduces, leads to a slowdown in the resorption of hemorrhages, the elimination of fibrin clots, detritus and small bone fragments. Even on the 10th day after using EMSCs in the postoperative wound a structureless detritus with a small number of infiltrating cells was present, as well as a significant number of multinucleated macrophages with fused cytoplasm, non-viable lysed striated muscle symplasts and bone fragments with a low degree of degradation. Conclusion. Suppression of inflammation by EMSCs delays the clearance of the postoperative wound, promotes the prolongation of the repair process and the attachment of the granulomatous component to the inflammation. The using EMSCs in the process of intraosseous implantation may be recommended only to control the activity of the inflammatory process and only after maximum preliminary cleansing of the postoperative wound from detritus, including non-viable muscle tissue and bone fragments. What this paper adds It has been firstly shown that after the experimental use of exosome of multipotent mesenchymal stromal cells to influence the regeneration of surgical trauma of soft tissues after intraosseous implantation, it is possible to reduce the activity of the inflammatory reaction, which leads to a slowdown of resorption of hemorrhages, elimination of fibrin clots, detritus and small bone fragments and, accordingly, prolongation of cleansing damaged tissue and delayed repair.
利用多能基质细胞的外泌体从碎屑中清洁受损组织的时间延长
客观的研究多能间充质基质细胞(EMSC)的外泌体对金属螺钉植入骨过程中受损软组织的影响。方法。在实验兔的胫骨近端髁上产生了一个缺损(直径2mm,深度4mm)。通过预先注射生理盐水(对照组,n=9只动物)或每条肢体19.2μg EMSC(实验组,n=10只兔子)将金属螺钉植入缺损处。手术后3、7和10天后,将动物从实验中取出;用光学显微镜对髁突表面软组织切片进行苏木精和伊红染色。后果在将金属植入物引入胫骨近端髁的过程中使用水冷却不会导致小骨碎片的完全去除,这些小骨碎片随后随着伤口排出而向外消除,或者被破坏并受到巨噬细胞的裂解。由于EMSC对损伤部位附近软组织的作用,术后炎症的活性降低,导致出血的吸收减慢,纤维蛋白凝块、碎屑和小骨碎片的消除。即使在术后伤口使用EMSC后的第10天,也存在具有少量浸润细胞的无结构碎屑,以及大量具有融合细胞质的多核巨噬细胞、不存活的溶解横纹肌症状和降解程度较低的骨碎片。结论EMSC抑制炎症可延迟术后伤口的清除,促进修复过程的延长以及肉芽肿成分与炎症的附着。建议在骨内植入过程中使用EMSC仅用于控制炎症过程的活性,并且仅在最大限度地初步清除术后伤口的碎屑(包括不活的肌肉组织和骨碎片)后使用。这篇论文的补充内容首次表明,在实验性使用多能间充质基质细胞的外泌体影响骨内植入后软组织手术创伤的再生后,可以降低炎症反应的活性,从而减缓出血的吸收,消除纤维蛋白凝块,碎屑和小骨碎片,因此,清洁受损组织的时间延长,修复延迟。
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来源期刊
Novosti Khirurgii
Novosti Khirurgii Medicine-Surgery
CiteScore
0.50
自引率
0.00%
发文量
15
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