Design of a miniaturized frequency domain near infrared spectrometer with validation in solid phantoms and human tissue

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-11-02 DOI:10.1177/09670335221134206

Alper Kılıç, Yun Miao, V. Koomson

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引用次数: 0

Abstract

Hemoglobin is one of the most important chromophores in the human body, since oxygen is carried to the tissue by binding with the hemoglobin. Therefore measuring the concentrations of oxy-hemoglobin (HbO) and deoxy-hemoglobin (HbR) is very important in both clinical settings and academic fields. Frequency domain near infrared spectroscopy (fdNIR spectroscopy) is a technique that can be used to measure the absolute concentrations of HbO and HbR non-invasively and locally. The fdNIR spectrometer utilizes the attenuation and the phase shift (with respect to the source) that an intensity modulated NIR light experiences in order to calculate the absorption (μa) and reduced scattering (μ′s) coefficient of the tissue. In this work, a miniaturized dual-wavelength fdNIR spectrometry instrument is presented with both tissue-like phantom and in vivo occlusion measurements. Systematic tests were performed on tissue phantoms to quantify the accuracy and stability of the instrument. The absolute errors for μa and μ′s were below 15% respectively. The amplitude and phase uncertainty were below 0.25% and 0.35°. In vivo measurements were also conducted to further validate the system.

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小型频域近红外光谱仪的设计，并在实体幻影和人体组织中进行验证

血红蛋白是人体中最重要的发色团之一，因为氧气通过与血红蛋白结合而被携带到组织中。因此，测量氧合血红蛋白（HbO）和脱氧血红蛋白（HbR）的浓度在临床和学术领域都非常重要。频域近红外光谱（fdNIR光谱）是一种可用于非侵入性和局部测量HbO和HbR绝对浓度的技术。fdNIR光谱仪利用强度调制的NIR光所经历的衰减和相移（相对于光源）来计算组织的吸收（μa）和减少的散射（μ′s）系数。在这项工作中，提出了一种小型化的双波长fdNIR光谱仪器，该仪器具有组织样体模和体内闭塞测量。对组织模型进行了系统测试，以量化仪器的准确性和稳定性。μa和μ′s的绝对误差分别小于15%。振幅和相位不确定度分别低于0.25%和0.35°。还进行了体内测量以进一步验证该系统。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.