Influences of the diurnal cycle on gut microbiota in the Chinese swamp shrimp (Neocaridina denticulata)

IF 1 4区生物学 Q3 BIOLOGY

Biological Rhythm Research Pub Date : 2022-07-29 DOI:10.1080/09291016.2022.2106711

Heng Zhang, Yingdong Li, Qing Liu

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引用次数: 0

Abstract

ABSTRACT The circadian rhythm is an essential environmental factor affecting several physiological activities in organisms, including crustaceans. However, daily variations in the gut bacterial community composition of crustaceans remain poorly understood. This study aimed to elucidate variations in the gut bacterial community composition of the Chinese swamp shrimp Neocaridina denticulata with the diurnal cycle. The gut samples of 600 shrimps were collected at four time points (6:00, 12:00, 18:00, and 24:00) within a 24 h period and 16S rRNA high-throughput gene sequencing was applied. The alpha-diversity and richness of the gut bacterial community were highest in the 24:00 group. Food digestion- and absorption-related pathways were highly enriched, indicating that metabolic process pathways are influenced by the daily cycle. Opportunistic pathogenic bacteria, including Flavobacterium, Acinetobacter, and Pseudomonas, were well-represented in the 18:00 and 24:00 groups, suggesting that the daily cycle shapes pathogenic bacterial community composition in the shrimp intestine. Our findings provide new insights into the role of diurnal cycles on gut bacteria in crustaceans and may aid in improving N. denticulata aquaculture.

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日循环对沼虾肠道菌群的影响

昼夜节律是影响生物(包括甲壳类动物)多种生理活动的重要环境因子。然而，甲壳类动物肠道细菌群落组成的日常变化仍然知之甚少。本研究旨在阐明中国沼泽虾(Neocaridina denticulata)肠道细菌群落组成随昼夜周期的变化。在24 h内的4个时间点(6:00、12:00、18:00和24:00)采集600只对虾的肠道样本，采用16S rRNA高通量基因测序。肠道细菌群落的α -多样性和丰富度在24:00组最高。与食物消化和吸收相关的途径高度丰富，表明代谢过程途径受到日常循环的影响。机会致病菌，包括黄杆菌、不动杆菌和假单胞菌，在18:00和24:00的组中有很好的代表性，这表明每天的周期决定了虾肠内致病菌的群落组成。我们的研究结果为昼夜循环对甲壳类动物肠道细菌的作用提供了新的见解，并可能有助于改善齿齿藻水产养殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biological Rhythm Research 生物-生理学

CiteScore

3.00

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The principal aim of Biological Rhythm Research is to cover any aspect of research into the broad topic of biological rhythms. The area covered can range from studies at the genetic or molecular level to those of behavioural or clinical topics. It can also include ultradian, circadian, infradian or annual rhythms. In this way, the Editorial Board tries to stimulate interdisciplinary rhythm research. Such an aim reflects not only the similarity of the methods used in different fields of chronobiology, but also the fact that many influences that exert controlling or masking effects are common. Amongst the controlling factors, attention is paid to the effects of climate change on living organisms. So, papers dealing with biometeorological aspects can also be submitted. The Journal publishes original scientific research papers, review papers, short notes on research in progress, book reviews and summaries of activities, symposia and congresses of national and international organizations dealing with rhythmic phenomena.